通过PI3K/Akt信号通路观察电针调节围绝经期肥胖脂代谢机制研究  

PI3K/Akt Signaling Pathway Mechanism of Electroacupuncture in Regulating Lipid Metabolism in Perimenopausal Obesity

作　　者：张超月 杨维 王照钦 李翔[1] 王祖庆 王宇晴 刘鑫 徐世芬[1] 宓轶群[1] 李珊珊 ZHANG Chaoyue;YANG Wei;WANG Zhaoqin;LI Xiang;WANG Zuqing;WANG Yuqing;LIU Xin;XU Shifen;MI Yiqun;LI Shanshan(Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China;Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China)

机构地区：[1]上海中医药大学附属市中医医院,上海200071 [2]上海市针灸经络研究所,上海200030

出　　处：《世界科学技术-中医药现代化》2024年第11期2791-2797,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：上海市科学技术委员会青年科技英才扬帆计划(21YF1444600):电针通过FSHR-FoxO1信号通路调控糖脂代谢对围绝经期肥胖的作用机制,负责人:李珊珊;国家自然科学基金青年项目(82305366):基于腺垂体miRNAs参与FSHβ表观遗传调控研究电针治疗围绝经期肥胖脂代谢紊乱的作用机制,负责人:李珊珊;上海市科学技术委员会上海自然科学基金项目(21ZR1461000):“委中”穴针刺对OAB大鼠的特异性效应及作用机制研究,负责人:宓轶群。

摘　　要：目的探讨电针关元穴对双侧卵巢去势(OVX)术诱导的围绝经期肥胖(PMO)大鼠肝脏组织磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/Akt)信号通路中PI3K、Akt、p-Akt蛋白的影响与调节脂代谢的潜在机制。方法将32只雌性SD大鼠随机分为空白组、假手术组、模型组和电针组,每组8只。模型组和电针组行OVX术制备PMO模型大鼠,假手术组仅摘除双侧卵巢旁等大脂肪。造模成功后,电针组电针关元穴,每天1次,连续14天。全程记录大鼠体质量,取材时计算胃腹围、Lee’s指数;HE染色观察肝脏病理变化;ELISA检测血清中HDL、LDL、TG水平;Western blot检测肝脏PI3K、Akt、p-Akt蛋白表达及p-Akt/Akt。结果与空白组、假手术组比较,模型组大鼠体质量、胃腹围、Lee’s指数升高(P<0.05),肝脏中出现脂肪空泡,血清HDL降低(P<0.001),LDL、TG升高(P<0.01),肝脏PI3K、Akt、p-Akt、p-Akt/Akt均降低(P<0.05);与模型组比较,电针组大鼠体质量、胃腹围、Lee’s指数下调(P<0.05),肝脏中脂肪空泡减少,HDL升高(P<0.01),LDL、TG降低(P<0.05,P<0.01),PI3K、Akt、p-Akt降低(P<0.05),p-Akt/Akt降低(P<0.01)。结论电针关元穴可上调PI3K/Akt信号通路,改善脂代谢紊乱,减轻OVX大鼠体质量。Objective To observe the expression of phosphatidylinositol 3-kinase(PI3K)and protein kinase B(Akt)signal transducer in liver tissue of rats with bilateral ovarian castration(OVX)by electroacupuncture at Guanyuan(CV4)point,and explore the mechanism of electroacupuncture in up regulating PI3K/Akt signal pathway,regulating lipid metabolism,and reducing weight was investigated.Methods Thirty-two female SD rats were randomly divided into blank,sham operation,model and electroacupuncture group,with 8 rats in each group.Perimenopausal obesity(PMO)model was prepared by OVX in the model and the electroacupuncture group.After the model was successfully prepared,the electroacupuncture group was subjected to electroacupuncture at CV4(continuous wave,2 Hz,1 mA),once day,20 min once time,and for 14 consecutive days.The body mass of each group of rats was recorded during the whole experiment,and Lee′s index was calculated during sampling.The pathological changes of liver were observed by HE staining.Serum levels of HDL,LDL and TG were detected by ELISA.Western blot analysis of PI3K,Akt,p-Akt protein expression and p-Akt/Akt in liver tissues.Results Compared with blank group and sham operation group,the body mass of rats in model group was significantly increased(P<0.05),Lee′s index was increased(P<0.05),fat vacuoles appeared in liver,serum HDL level was decreased(P<0.001),LDL and TG levels were increased(P<0.01).PI3K,Akt,p-Akt,p-Akt/Akt were decreased in liver(P<0.05).Compared with model group,body mass and Lee′s index of rats in electroacupuncture group were decreased(P<0.05),fat vacuoles in liver were decreased,HDL level was increased(P<0.01),LDL and TG levels were decreased(P<0.05,P<0.01),PI3K,Akt and P-Akt were decreased(P<0.05),and p-akt/Akt decreased(P<0.01).Conclusion Electroacupuncture at CV4 can significantly up-regulate PI3K/Akt signaling pathway,improve lipid metabolism disorder and accumulation,and reduce body mass in OVX rats.

关 键 词：电针 围绝经期肥胖 脂代谢 PI3K/AKT信号通路 双侧卵巢去势 

分 类 号：R245.97[医药卫生—针灸推拿学]