宁夏回族MNS、Lutheran、Kell红细胞血型系统基因频率调查研究  

Investigation on gene frequencies of MNS,Lutheran and Kell erythrocyte blood group system in Ningxia Hui nationality

作　　者：步晓筠[1] BU Xiaoyun(Blood Group Reference Laboratory,Blood Center of Ningxia Hui Autonomous Region,Yinchuan,Ningxia 750004,China)

机构地区：[1]宁夏回族自治区血液中心血型参比实验室,宁夏银川750004

出　　处：《检验医学与临床》2025年第3期340-344,共5页Laboratory Medicine and Clinic

基　　金：宁夏回族自治区卫生健康系统科研课题(2022-NWKY-041)。

摘　　要：目的探究宁夏回族MNS、Lutheran、Kell红细胞血型系统主要抗原基因频率的分布特征。方法采用实时荧光定量聚合酶链反应对140份宁夏回族MNS、Lutheran、Kell红细胞稀有血型系统抗原基因进行分型和多态性分析。结果宁夏回族MNS、Lutheran及Kell血型系统基因呈多态性分布。各血型系统基因型分布的观察值与期望值比较,差异均无统计学意义(P>0.05)。符合Hardy-Weinberg定律,宁夏回族的血型基因频率在世代间保持相对稳定。血型抗原对偶抗原不配合率由高至低分别为M/N抗原(37.37%)、S/s抗原(12.91%)、K/k抗原(1.41%)、Lu a/Lu b抗原(0.71%)。宁夏回族与内蒙古蒙古族、海南黎族、新疆哈萨克族、西藏藏族及四川彝族M和N等位基因频率比较,差异均有统计学意义(P<0.05);宁夏回族与海南黎族、新疆哈萨克族、新疆维吾尔族、西藏藏族S和s等位基因频率比较,差异均有统计学意义(P<0.05)。宁夏回族Lutheran血型系统Lu a和Lu b等位基因与其他民族比较,差异均无统计学意义(P>0.05),但其他民族均为Lu b纯合子,宁夏回族中发现Lu a等位基因少量分布,宁夏回族Lutheran血型系统等位基因频率具有自身分布特点。宁夏回族Kell血型系统K和k等位基因频率与不同民族比较,差异均无统计学意义(P>0.05)。结论宁夏回族MNS、Lutheran、Kell血型系统基因呈多态性分布。宁夏回族MNS抗原不配合率较高,有必要在该地开展MNS血型抗原筛选,对孕产妇及反复多次输血者开展精准输血,避免不规则抗体导致胎儿新生儿溶血病、交叉配血困难延误救治情况的发生。Objective To explore the distribution characteristics of major antigen gene frequencies of MNS,Lutheran and Kell erythrocyte blood group system in Ningxia Hui nationality.Methods The MNS,Lutheran and Kell rare blood group system antigen genes of 140 cases of Ningxia Hui nationality were typed and analyzed by real-time fluorescence quantitative polymerase chain reaction.Results The allele frequency distribution of three rare blood group systems in Hui nationality in Ningxia region,and the MNS,Lutheran and Kell blood group system genes show polymorphism distribution.The MNS,Lutheran and Kell blood group system genes of Ningxia Hui nationality show polymorphism distribution.There was no significant difference between the observed value and the expected value of genotype distribution in each blood group system(P>0.05).In accordance with Hardy-Weinberg law,the blood group gene frequency of Ningxia Hui population remained relatively stable between generations.The mismatched rate of blood group antigen pairs from high to low was M/N(37.37%),S/s(12.91%),K/k(1.41%),Lu a/Lu b(0.71%)respectively.There were significant differences in the frequencies of M and N alleles between Ningxia Hui and Inner Mongolia,Hainan Li,Xinjiang Kazak,Tibet and Sichuan Yi(P<0.05).There were significant differences in the frequencies of S and s alleles between Ningxia Hui and Hainan Li,Xinjiang Kazak,Xinjiang Uygur,and Tibet(P<0.05).There was no significant difference in Lu a and Lu b alleles between Ningxia Hui and other ethnic groups(P>0.05),but other ethnic groups were Lu b homozygous.Lu a allele was found in Ningxia Hui,and the allele frequency of Ningxia Hui Lutheran blood group system has its own distribution characteristics.There was no significant difference in the allele frequency of Kell blood group system K and k between Ningxia Hui nationality and other nationalities(P>0.05).Conclusion The MNS,Lutheran and Kell blood group system genes in Ningxia Hui are polymorphic.The rate of MNS antigen non-compliance in Ningxia Hui is high.It is n

关 键 词：红细胞血型 稀有血型 基因频率 回族 输血 

分 类 号：R457.1[医药卫生—治疗学] R392.7[医药卫生—临床医学]