大豆苷元对心肌梗死大鼠心电图、心肌肌钙蛋白I及Raf-MEK-ERK信号通路的影响  

Effects of daidzein on electrocardiogram,cardiac troponin I and Raf-MEK-ERK signaling pathway in rats with myocardial infarction

作　　者：李会贤[1] 张爱爱[1] 张亚维 陈伟广 马骁 梁惠清[1] LI Huixian;ZHANG Aiai;ZHANG Yawei;CHEN Weiguang;MA Xiao;LIANG Huiqing(Department of Cardiovascular Medicine,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China;Zhangjiakou Health Commission of Hebei Province,Zhangjiakou,Hebei 075000,China;Department of Inpatient,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China)

机构地区：[1]河北北方学院附属第一医院心血管内科,河北张家口075000 [2]河北省张家口市卫生健康委员会,河北张家口075000 [3]河北北方学院附属第一医院住院处,河北张家口075000

出　　处：《检验医学与临床》2025年第3期377-383,共7页Laboratory Medicine and Clinic

基　　金：河北省医学科学研究基金项目(20231406);河北省卫生健康委员会医学科学研究项目(20200538)。

摘　　要：目的探讨大豆苷元对心肌梗死大鼠心电图、心肌肌钙蛋白I(cTnI)及Raf-MEK-ERK信号通路的影响。方法取无特定病原体等级成年雄性大鼠60只作为研究对象。将60只大鼠随机分为假手术组、模型组、低剂量大豆苷元组、中剂量大豆苷元组、高剂量大豆苷元组及瑞舒伐他汀组,每组10只。除假手术组外均建立心肌梗死动物模型。监测各组大鼠心电参数;采用酶联免疫吸附试验检测各组血清cTnI、肌酸激酶同工酶(CK-MB)、肿瘤坏死因子(TNF)-α及白细胞介素(IL)-6水平;采用苏木精-伊红染色观察心肌组织病理形态;采用TUNEL法检测心肌细胞凋亡率;采用免疫印记检测心肌组织中Raf、MEK、ERK蛋白水平。结果与假手术组比较,模型组和低、中、高剂量大豆苷元组及瑞舒伐他汀组T波均升高,差异均有统计学意义(P<0.05)。与假手术组比较,模型组和低、中、高剂量大豆苷元组及瑞舒伐他汀组cTnI、CK-MB、TNF-α及IL-6水平均升高,差异均有统计学意义(P<0.05);与模型组比较,低、中、高剂量大豆苷元组及瑞舒伐他汀组cTnI、CK-MB、TNF-α及IL-6水平均降低,差异均有统计学意义(P<0.05)。假手术组、模型组和低、中、高剂量大豆苷元组及瑞舒伐他汀组大鼠心肌细胞凋亡率分别为(3.56±0.41)%、(32.50±5.50)%、(24.15±3.66)%、(19.66±2.80)%、(11.20±1.80%)及(12.03±2.11)%。与假手术组比较,模型组和低、中、高剂量大豆苷元组及瑞舒伐他汀组心肌细胞凋亡率升高,差异均有统计学意义(P<0.05);与模型组比较,低、中、高剂量大豆苷元组及瑞舒伐他汀组心肌细胞凋亡率均降低,差异均有统计学意义(P<0.05)。与假手术组比较,模型组和低、中、高剂量大豆苷元组及瑞舒伐他汀组Raf、MEK、ERK蛋白水平均升高,差异均有统计学意义(P<0.05);与模型组比较,低、中、高剂量大豆苷元组及瑞舒伐他汀组Raf、MEK、ERK蛋白水平均降低,差异均�Objective To investigate the effects of daidzein on electrocardiogram,cardiac troponin I(cTnI)and Raf-MEK-ERK signaling pathway in rats with myocardial infarction.Methods Sixty specific pathogen-free adult male rats were selected as the study subjects.Sixty rats were randomly divided into sham operation group,model group,low dose daidzein group,medium dose daidzein group,high dose daidzein group and rosuvastatin group,with 10 rats in each group.Animal models of myocardial infarction were established except for the sham operation group.Electrocardiogram parameters were monitored in each group.Enzyme-linked immunosorbent assay was used to detect the serum levels of cTnI,creatine kinase isoenzyme(CK-MB),tumor necrosis factor(TNF)-α and interleukin(IL)-6.Hematoxylin-eosin staining was used to observe the pathological morphology of myocardial tissue.The apoptosis rate of cardiomyocytes was detected by TUNEL method.The protein levels of Raf,MEK and ERK in myocardial tissue were detected by Western blot.Results Compared with the sham operation group,the model group,low dose daidzein group,medium dose daidzein group and high and rosuvastatin group had significant increases in T wave(P<0.05).Compared with the sham operation group,the levels of cTnI,CK-MB,TNF-α and IL-6 in the model group,low dose daidzein group,medium dose daidzein group,high dose daidzein group and rosuvastatin group were increased,the differences were statistically significant(P<0.05),compared with the model group,the levels of cTnI,CK-MB,TNF-α and IL-6 in the low,medium and high dose daidzein groups and rosuvastatin group were decreased,and the differences were statistically significant(P<0.05).The apoptosis rates of myocardial cells in the sham operation group,model group,low dose daidzein group,medium dose daidzein group,high dose daidzein group and rosuvastatin group were(3.56±0.41)%,(32.50±5.50)%,(24.15±3.66)%,(19.66±2.80)%,(11.20±1.80)% and(12.03±2.11)%respectively.Compared with the sham operation group,the apoptosis rate of myocardial ce

关 键 词：心肌梗死 大豆苷元 心肌肌钙蛋白I 心电图 Raf-MEK-ERK 

分 类 号：R542.2[医药卫生—心血管疾病] R541.7[医药卫生—内科学]