活动性肺结核患者血清miR-708、miR-99a水平及临床意义  

Serum levels and clinical significance of miR-708 and miR-99a in patients with active pulmonary tuberculosis

作　　者：刘红 徐瑞芳 洪伟俊 LIU Hong;XU Ruifang;HONG Weijun(Department of Infectious Diseases,Central Hospital of Minhang District,Shanghai 201199,China;Department of Respiratory Medicine,Central Hospital of Minhang District,Shanghai 201199,China)

机构地区：[1]上海市闵行区中心医院感染科,上海201199 [2]上海市闵行区中心医院呼吸内科,上海201199

出　　处：《检验医学与临床》2025年第3期410-414,共5页Laboratory Medicine and Clinic

基　　金：上海市浦东新区卫生系统学科建设项目(PWZxk2022-24)。

摘　　要：目的探讨活动性肺结核(APTB)患者血清微小核糖核酸-708(miR-708)、微小核糖核酸-99a(miR-99a)水平及临床意义。方法选取2020年6月至2021年6月该院收治的APTB患者94例作为APTB组,另选取同期于该院体检中心进行体检的健康志愿者94例作为对照组。根据APTB患者病情严重程度分为轻度组、中度组、重度组。根据预后结果分为预后不良组、预后良好组。采用实时荧光定量聚合酶链反应检测血清miR-708、miR-99a的相对表达量。采用Spearman相关分析miR-708、miR-99a水平与疾病严重程度的关系。绘制受试者工作特征(ROC)曲线分析血清miR-708、miR-99a单独及联合检测对APTB预后的评估价值。结果APTB组血清miR-708、miR-99a水平均低于对照组,差异均有统计学意义(P<0.05)。重度组纳入31例,中度组纳入30例,轻度组纳入33例。轻度组血清miR-708、miR-99a水平均高于中度组与重度组,且中度组血清miR-708、miR-99a水平均高于重度组,差异均有统计学意义(P<0.05)。Spearman相关分析结果显示,miR-708、miR-99a水平与疾病严重程度均呈负相关(r s=-0.687,P<0.05;r s=-0.523,P<0.05)。预后良好组纳入60例,预后不良组纳入34例。预后不良组血清miR-708、miR-99a水平均明显低于预后良好组,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,血清miR-708对APTB预后评估的曲线下面积(AUC)为0.883,血清miR-99a对APTB预后评估的AUC为0.909,二者联合检测的AUC为0.957,与miR-708、miR-99a单独检测比较,二者联合检测的AUC更大(Z=6.601、10.832,P<0.001)。结论APTB患者血清miR-708、miR-99a水平降低,二者与APTB患者病情严重程度及预后有关,可以作为APTB预后评估的辅助生物学标志物。Objective To investigate the levels and clinical significance of serum microRNA-708(miR-708)and microRNA-99a(miR-99a)in patients with active pulmonary tuberculosis(APTB).Methods A total of 94 APTB patients admitted to the hospital from June 2020 to June 2021 were selected as the APTB group,and 94 healthy volunteers who underwent physical examination in the physical examination center of the hospital during the same period were selected as the control group.According to the severity of APTB,the patients were divided into mild group,moderate group and severe group.According to the prognosis results,the patients were divided into poor prognosis group and good prognosis group.The relative expression levels of serum miR-708 and miR-99a were detected by real-time fluorescence quantitative polymerase chain reaction.Spearman correlation was used to analyze the relationship between the levels of miR-708 and miR-99a and the severity of the disease.The receiver operating characteristic(ROC)curve was drawn to analyze the value of serum miR-708,miR-99a alone and combined detection in evaluating the prognosis of APTB.Results The serum levels of miR-708 and miR-99a in the APTB group were lower than those in the control group,and the differences were statistically significant(P<0.05).Thirty-one patients were included in the severe group,thirty patients in the moderate group,and thirty-three patients in the mild group.The serum levels of miR-708 and miR-99a in the mild group were higher than those in the moderate group and the severe group,and the serum levels of miR-708 and miR-99a in the moderate group were higher than those in the severe group,and the differences were statistically significant(P<0.05).Spearman correlation analysis showed that the levels of miR-708 and miR-99a were negatively correlated with the severity of the disease(r s=—0.687,P<0.05,r s=—0.523,P<0.05).There were 60 patients in the good prognosis group and 34 patients in the poor prognosis group.The levels of serum miR-708 and miR-99a in the poor prognos

关 键 词：活动性肺结核 微小核糖核酸-708 微小核糖核酸-99a 预后 

分 类 号：R521[医药卫生—内科学] R445[医药卫生—临床医学]