Large-scale production of rice haploids by combining superior haploid inducer with PTGMS lines  

作　　者：Chaolei Liu Song Yan Fangming Mao Tingting Sun Huan Liang Qing Liu Qian Qian Kejian Wang 

机构地区：[1]State Key Laboratory of Rice Biology and Breeding,China National Rice Research Institute,Hangzhou 310006,China [2]Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China [3]Yazhouwan National Laboratory,Sanya 572024,China [4]Hainan Seed Industry Laboratory,Sanya 572025,China

出　　处：《Plant Communications》2024年第12期1-4,共4页植物通讯(英文)

基　　金：National Natural Science Foundation of China(32188102,32372177,32025028,and 2023YFD1200800);Central Public-interest Scientific Institution Basal Research Fund(Y2022QC20).

摘　　要：Dear Editor,Doubled haploid(DH)technology is the fastest and most efficient method for producing completely homozygous lines,commonly used in maize breeding(Ren et al.,2017).The discovery of Stock 6,the first maize haploid inducer(HI)reported in 1959,yields 2%–3%maternal haploids during self-pollination or when used as a male in outcrossing.High-efficiency HIs derived from Stock 6,with a haploid induction rate(HIR)of 7%–15%,have significantly contributed to the commercialization of maize DH breeding.Recently,the genetic basis of haploid induction has been identified with two main molecular players in maize:MTL/ZmPLA1/NLD,which triggers haploid production(Gilles et al.,2017;Kelliher et al.,2017;Liu et al.,2017),and DMP,which enhances haploid induction(Zhong et al.,2019).Importantly,MTL is conserved across cereals,and knockout of OsMATL in rice has produced 2%–6%haploids(Yao et al.,2018;Wang et al.,2019;Liu et al.,2024a),presenting promising prospects for rice breeding.However,due to the lack of high-efficiency HIs and limitations in cross-pollination,DH technology based on Osmatl remains an unreliable and inefficient practice for rice breeding.Here,we developed a high-efficiency HI with favorable agronomic performance in rice.We also present a large-scale haploid production approach achieved by combining this HI with photo-thermo-sensitive genic male sterile(PTGMS)lines(Figure 1A).

关 键 词：STERILE BREEDING HAPLOID 

分 类 号：S511[农业科学—作物学]