AcRLK2P-1,an LRR receptor protein kinase gene from Agropyron cristatum,confers leaf rust resistance in wheat  

作　　者：Shirui Xu Xiajie Ji Haiming Han Jinpeng Zhang Shenghui Zhou Baojin Guo Xinming Yang Xiuquan Li Xiaomin Guo Taiguo Liu Lihui Li Weihua Liu 

机构地区：[1]State Key Laboratory of Crop Gene Resources and Breeding,Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization(MARA),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(ICS-CAAS),Beijing 100081,China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China

出　　处：《Plant Communications》2024年第12期9-12,共4页植物通讯(英文)

基　　金：National Natural Science Foundation of China(32272083).

摘　　要：Dear Editor,Wheat(Triticum aestivum L.)is the most widely cultivated staple food crop globally.Wheat leaf rust,caused by Puccinia triticina,represents one of the most prevalent and devastating diseases affecting wheat,causing yield losses of up to 50%in severe infestations(Lin et al.,2022).To date,a total of 11 leaf rust resistance genes have been cloned,however,Lr1,Lr10,and Lr14a alone have proven are largely ineffective in wheat breeding programs in China(Zhang et al.,2020;Li et al.,2023).Previous studies have demonstrated the broad-spectrum resistance of the wheat–Agropyron cristatum translocation line 2PT5 to 50 leaf rust races from various regions across China,and the leaf rust resistance gene is located on a segment of 2PL bin fragment length(FL)0.66–0.86(Jiang et al.,2018).Given the scarcity of broad-spectrum leaf rust resistance genes in current wheat cultivars,it is crucial to explore and clone these genes from A.cristatum 2PL for their effective integration into breeding programs aimed at developing disease-resistant cultivars.The lack of genetic recombination between the alien segment and the corresponding wheat genome makes narrowing down the target region a challenging task.In this study,we employed PacBio isoform sequencing technology in conjunction with RNA sequencing to clone the leaf rust resistance gene AcRLK2P-1 from the wheat–A.cristatum 2PL translocation fragment.We further validated the functionality of AcRLK2P-1 through EMS mutant analysis and transgenic experiments.

关 键 词：CULTIVAR BREEDING WHEAT 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]