Fine-tuning of the dual-role transcription factor WRKY8 via differential phosphorylation for robust broad-spectrum plant immunity  

作　　者：Chun-Xiu Ren Song-Yu Chen Yu-Han He You-Ping Xu Juan Yang Xin-Zhong Cai 

机构地区：[1]Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects,Institute of Biotechnology,College of Agriculture and Biotechnology,Zhejiang University,866 Yu Hang Tang Road,Hangzhou 310058,China [2]Centre of Analysis and Measurement,Zhejiang University,866 Yu Hang Tang Road,Hangzhou 310058,China [3]Hainan Institute,Zhejiang University,Sanya 572025,China

出　　处：《Plant Communications》2024年第12期91-109,共19页植物通讯(英文)

基　　金：National Natural Science Foundation of China(no.31871947);Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding(no.2021C02064);Hainan Provincial Natural Science Foundation of China(no.324CXTD430);Zhejiang Provincial Natural Science Foundation of China(no.LZ18C140002).

摘　　要：Plants perceive pathogen-associated molecular patterns(PAMPs)using plasma-membrane-localized pattern recognition receptors(PRRs)to activate broad-spectrum pattern-triggered immunity.However,the regulatory mechanisms that ensure robust broad-spectrum plant immunity remain largely unknown.Here,we reveal that the transcription factor WRKY8 has a dual role in the transcriptional regulation of PRR genes:repressing expression of the nlp20/nlp24 receptor gene RLP23 while promoting that of the chitin receptor gene CERK1.SsNLP1 and SsNLP2,two nlp24-type PAMPs from the destructive fungal pathogen Sclerotinia sclerotiorum,activate two calcium-elicited kinases,CPK4 and CPK11,which phosphorylate WRKY8 and thus release its inhibition on RLP23 to promote accumulation of RLP23 transcripts.Meanwhile,SsNLPs activate the RLCK-type kinase PBL19,which phosphorylates WRKY8 and thus enhances accumulation of CERK1 transcripts.Intriguingly,RLP23 is repressed at later stage by PBL19-mediated phosphorylation of WRKY8,thus avoiding excessive immunity and enabling normal growth.Our findings unveil a plant strategy of“killing two birds with one stone”to elicit robust broad-spectrum immunity.This strategy is based on PAMP-triggered fine-tuning of a dual-role transcription factor to simultaneously amplify two PRRs that recognize PAMPs conserved across a wide range of pathogens.Moreover,our results reveal a novel plant strategy for balancing the trade-off between growth and immunity by fine-tuning the expression of multiple PRR genes.

关 键 词：WRKY8 RLP23 CERK1 calcium-dependent protein kinases PBL19 plant immunity Sclerotinia sclerotiorum 

分 类 号：Q94[生物学—植物学]