Antibacterial and cytotoxicity activity of green synthesized silver nanoparticles using aqueous extract of naartjie(Citrus unshiu)fruit peels  

作　　者：Livhuwani Mafhala Nomcebo Khumalo Nkosingiphile Excellent Zikalala Shohreh Azizi Karen Jacqueline Cloete Garland Kgosi More Ilunga Alain Kamika Touhami Mokrani Ali Akbar Zinatizadeh Malik Maaza 

机构地区：[1]UNESCO-UNISA Africa Chair in Nanoscience and Nanotechnology College of Graduates Studies,University of South Africa,Muckleneuk Ridge,Pretoria,392,South Africa [2]Nanosciences African Network(NANOAFNET)-Materials Research Department,iThemba LABS-National Research Foundation,Somerset West,P.O.Box 722,Cape Town 7129,South Africa [3]Department of Environmental Sciences,College of Agriculture and Environmental Sciences,University of South Africa,Private Bag X06,Florida 1710,South Africa [4]Institute of Nanotechnology and Water Sustainability,School of Science,College of Science,Engineering,and Technology,Florida Campus,University of South Africa,P.O Box 392,Florida,Roodepoort,1709,South Africa [5]College of Agriculture and Environmental Sciences Laboratories,University of South Africa,Private Bag X06,Florida,Johannesburg,1710,South Africa [6]Department of Chemical Engineering,University of South Africa,Private Bag X6,Florida,Johannesburg,1709,South Africa [7]Environmental Research Center,Department of Applied Chemistry,Razi University,Kermanshah P.O.Box 67144-14971,Iran

出　　处：《Emerging Contaminants》2024年第4期153-163,共11页新兴污染物(英文)

基　　金：funding from South African Coal,Oil,and Gas Corporation-National Research Foundation(SASOL-NRF)Uni-versity Collaborative Research Grants(Grant No.138626).

摘　　要：The application of plant extracts in the green synthesis of silver nanoparticles(AgNPs)has attracted significant attention owing to their eco-friendliness and cost-effective features.However,nanoparticles synthesized from plant materials may also display unique physicochemical properties.This study described an improved synthesis process for silver nanoparticles(AgNPs)from the peel of the Citrus unshiu fruit,an agricultural waste product.In addition to evaluating the produced AgNPs'physico-chemical characteristics,the research also examines their antibacterial and cytotoxic/anticancer capa-bilities.The color change from yellow to dark brown was observed immediately when 20 ml of Citrus unshiu fruit peel extract was added to 0.5 g of silver nitrate.A surface plasmon resonance band at 424 nm revealed by ultra-violet spectroscopy was used as additional confirmation that AgNP had formed.The scanning electron microscope(SEM),and the transmission electron microscope(TEM),revealed spherical-shaped NPs with a mean size of 23 nm.Furthermore,X-ray diffraction(XRD)confirmed the high crystallinity of the obtained NPs while Fourier transform infrared spectroscopy(FTIR)confirmed the bioreducing capacity of the Citrus unshiu fruit peel extract.The minimum inhibitory concentration(MIC)(31.25 mg/mL)of AgNPs significantly revealed a strong dose-dependent antibacterial effect on Staphy-lococcus aureus and Bacillus cereus.In contrast,their impact on gram-negative bacteria,such as Escher-ichia coli and Salmonella typhimurium,displayed an MIC of 250 mg/mL.The assessment of cytotoxicity on human embryonic kidney cells(HEK293)and lung cancer cells(A549)through a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay revealed no detectable cytotoxic effects.The IC 50 values were determined as 37.73±0.34 mg/mL for HEK293 and 56.37±0.73 mg/mL for A549,indicating the absence of significant adverse impacts on these cell lines.The anticancer drug doxorubicin was used as a reference standard,and had an IC 50 value of 1.22±0.09

关 键 词：Antimicrobial activity Citrus unshiu Cytotoxicity activity Green synthesis Silver nanoparticle 

分 类 号：O62[理学—有机化学]