生物炭强化硫自养反硝化过程中亚硝酸盐的积累  

Biochar enhanced nitrite accumulation during sulfur autotrophic denitrification

作　　者：孙嘉炜 杨彪 王仲宇 端允[1] SUN Jiawei;YANG Biao;WANG Zhongyu;DUAN Yun(School of Environmental Science and Engineering,Taiyuan University of Technology,Jinzhong 030600)

机构地区：[1]太原理工大学环境科学与工程学院,晋中030600

出　　处：《环境科学学报》2025年第1期32-38,共7页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金面上项目(No.52170045)。

摘　　要：硫自养反硝化(SADN)可为厌氧氨氧化(Anammox)提供亚硝酸盐(NO_(2)^(-)-N).为了促进SADN过程的NO_(2)^(-)-N积累,本实验以S^(0)为电子供体,考察了污泥-煤矸石基生物炭(SCBC)对SADN中NO_(2)^(-)-N积累率及抗冲击能力的影响.实验结果表明,投加SCBC使SADN的NO_(3)^(-)-N还原速率由70.6 mg·L^(-1)·h^(-1)提升至107.7 mg·L^(-1)·h^(-1),NO_(2)^(-)-N还原速率由30.5 mg·L^(-1)·h^(-1)提升至35.1 mg·L^(-1)·h^(-1),这使得NO_(2)^(-)-N积累率由48.2%提升至63.0%.当遇到冲击负荷(300 mg·L^(-1)的NO_(3)^(-)-N)时,SADN的还原性能下降,6 d后逐步恢复并继续提高,不投加则需12 d才能恢复.投加SCBC 60 d后,PN/PS和PN值分别为3.51和5.27 mg·g^(-1),高于不投加的2.36和3.44 mg·g^(-1).高通量测序均能检测到反硝化菌Thiobacillus、Sulfurimonas和Thermomonas,投加SCBC对反硝化菌起到富集作用,使其占比分别由40.0%、0.61%、0.03%上升为51.8%、2.71%、3.47%.总之,添加SCBC为提高SADN的NO_(2)^(-)-N积累提供了一个新方法,这也为污泥和煤矸石的资源化利用提供了一种新的途径.Sulfur autotrophic denitrification (SADN) can provide NO_(2)^(-)-N for anaerobic ammonia oxidation (Anammox).In order to promote the accumulation of NO_(2)^(-)-N in SADN process,this study used S^(0) as the electron donor and investigated the effects of sludge/coal gangue-derived biochar(SCBC) on the NO_(2)^(-)-N accumulation rate and shock resistance in SADN system.The results illustrated that the addition of SCBC increased the NO_(3)^(-)-N reduction rate in SADN from 70.6 mg·L^(-1)·h^(-1) to 107.7 mg·L^(-1)·h^(-1),and the NO_(2)^(-)-N reduction rate increased from 30.5 mg·L^(-1)·h^(-1) to 35.1 mg·L^(-1)·h^(-1),resulting in an increase in the NO_(2)^(-)-N accumulation rate from 48.2%to 63.0%.When subjected to a shock load of 300 mg·L^(-1 )NO_(3)^(-)-N,the reduction performance of SADN initially decreased,but recovered gradually after 6 days,and continued to increase,compared to12 days without the addition of SCBC.After 60 days,the PN/PS and PN values were 3.51 and 5.27 mg·g^(-1),respectively,higher than those without SCBC addition,which were 2.36and 3.44 mg·g^(-1),respectively.The denitrifying bacteria Thiobacillus,Sulfurimonas,and Thermomonas were detected by high-throughput sequencing analysis.SCBC supplementation enhanced these bacteria,increasing their proportions from 40.0%,0.61%,and 0.03%to 51.8%,2.71%,and 3.47%,respectively.In conclusion,SCBC addition presents a new avenue for NO_(2)^(-)-N accumulation in SADN treatment,while also offering a novel strategy for sludge and coal gangue utilization.

关 键 词：硫自养反硝化 厌氧氨氧化 污泥煤矸石基生物炭 亚硝酸盐积累 强化 

分 类 号：X705[环境科学与工程—环境工程] X703