光助漂浮型铜钼双金属氮化碳复合材料活化PMS去除水中抗生素抗性菌与抗性基因的性能及机理  

Study on the performance and mechanism of ARB and ARGs removal in wastewater by Vis/Cu^(0)-MoS_(2)/C_(3)N_(4)-EG enhanced peroxymonosulfate activation process

作　　者：陈曦 刘熠阳 周见卓 王官禄 王学江[1] CHEN Xi;LIU Yiyang;ZHOU Jianzhuo;WANG Guanlu;WANG Xuejiang(State Key Laboratory of Pollution Control and Resource Reuse,College of Environmental Science and Engineering,Tongji University,Shanghai 200082)

机构地区：[1]同济大学环境科学与工程学院,污染控制与资源化研究重点实验室,上海200082

出　　处：《环境科学学报》2025年第1期97-110,共14页Acta Scientiae Circumstantiae

基　　金：国家重点研发计划项目(No.2021YFC3201302)。

摘　　要：通过光还原法制备了一种漂浮型Cu^(0)和MoS_(2)双金属氮化碳复合材料Cu^(0)-MoS_(2)/C_(3)N_(4)-EG,构建了可见光助Cu^(0)-MoS_(2)/C_(3)N_(4)-EG活化PMS体系(Vis/Cu^(0)-MoS_(2)/C_(3)N_(4)-EG/PMS)用于去除水中抗生素抗性菌(ARB)和抗性基因(ARGs).灭菌实验结果表明,Vis/Cu^(0)-MoS_(2)/C_(3)N_(4)-EG/PMS体系在材料投加量为0.22 g·L^(-1),PMS浓度为1 g·L^(-1)时,仅需0.5 min即可灭活全部TC-ARB(初始浓度为5.4×10~7 CFU·mL^(-1)).灭菌过程能够破坏细胞膜的结构,进而导致DNA片段泄露并受到ROS的攻击,使得ARGs的水平转移频率降低;淬灭实验和ESR实验结果表明,起主要作用的活性物质是·OH、·SO_(4)^(-)和^(1)O_(2).Vis/Cu^(0)-MoS_(2)/C_(3)N_(4)-EG/PMS体系对实际污水处理厂二沉池出水消毒处理结果表明,该体系能够在2 min内完成高效消毒,并且大幅降低水中各类ARGs的绝对丰度.研究结果为TC-ARB及其ARGs污染的同步高效去除提供了技术支撑和理论依据.In this study,a floating copper-molybdenum bimetallic carbon nitride composite Cu^(0)-MoS_(2)/C_(3)N_(4)-EG was synthesized.A visible-light-assisted Cu^(0)-MoS_(2)/C_(3)N_(4)-EG activated PMS system(Vis/Cu^(0)-MoS_(2)/C_(3)N_(4)-EG/PMS)was developed for the removal of antibiotic-resistant bacteria(ARB)and antibiotic resistance genes(ARGs)from water.The results showed that this system could inactivate all tetracycline-resistant antibiotic-resistant bacteria(TC-ARB)within only 0.5 min,starting from an initial concentration of 5.4×10^(7) CFU·mL^(-1),using a catalyst dosage of 0.22 g·L^(-1)and PMS dosage of 1 g·L^(-1).This system could destroy the cell membrane of TC-ARB,with the leaked DNA fragments further attacked by reactive oxygen species(ROS),which significantly reduce the horizontal transfer of TC-ARGs.Quenching test and electron paramagnetic resonance(EPR)analyses showed that the main active species included·OH,·SO_(4)^(-) and ^(1)O_(2).For secondary effluent from an actual sewage treatment plant,this system achieved efficient disinfection within 2 minutes and greatly reduce the absolute abundance of various ARGs in the water.This study provide technical support and theoretical basis for the removal of TC-ARB and ARGs from whater.

关 键 词：过硫酸盐活化 双金属催化剂 抗生素抗性基因 可见光 氮化碳 

分 类 号：X703[环境科学与工程—环境工程]