藻华生消过程对海水铜络合容量的影响  

Effects of algal bloom growth and decay on the copper complexing capacity of seawater

作　　者：姜翰林 周立敏[1] 陈静 张天姿 曹晓燕[1,2] JIANG Hanlin;ZHOU Limin;CHEN Jing;ZHANG Tianzi;CAO Xiaoyan(Key Laboratory of Marine Chemistry Theory and Technology,Ministry of Education,College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100;Laboratory for Marine Ecology and Environmental Science,Qingdao Marine Science and Technology Center,Qingdao 266237)

机构地区：[1]中国海洋大学,化学化工学院,海洋化学理论与工程技术教育部重点实验室,青岛266100 [2]青岛海洋科技中心海洋生态与环境科学功能实验室,青岛266237

出　　处：《环境科学学报》2025年第1期188-200,共13页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.41976137,41676064,42330402)。

摘　　要：以我国近海藻华优势种中肋骨条藻(Skeletonema costatum)、东海原甲藻(Prorocentrum donghaiense)和浒苔(Ulva prolifera)为研究对象,通过室内培养实验、近岸绿潮区的围隔培养和现场调查,利用阳极溶出伏安法和分子光谱学技术分析了藻华生消过程中重金属有机配体的释放规律和组成变化特征.结果表明,中肋骨条藻和东海原甲藻生消过程中有机配体的大量释放发生在生长初期和消亡期,在指数生长期释放较慢;浒苔暴发期产生的有机配体促使围隔内铜络合容量平均值为围隔外的2.82倍,现场数据显示浒苔的聚积腐烂导致近岸绿潮区铜络合容量相较浒苔靠岸前提高37.81%.藻华生长阶段铜络合容量的增长与浮游植物直接分泌的类蛋白质等不稳定溶解有机质组分有关,在生长期结束则归因于腐殖质类物质等大分子惰性副产物.亚致死量的铜胁迫加快了浒苔藻体降解和有机配体的释放,铜胁迫下浒苔络合容量为对照组的2.78倍,体系中的游离铜离子被有效络合,至培养结束仅为初始的6.27%,显著降低了其生物毒性.The release of copper-complexing ligands during algae growth and decay was studied by anodic stripping voltammetry and molecular spectroscopy techniques through simulation experiments and field investigations.The algae included Skeletonema costatum,Prorocentrum donghaiense,and Ulva prolifera,which are the common predominant species of algal blooms in the coastal areas of China.The simulation experiments results showed significant release of organic ligands during the initial and decay periods of Skeletonema costatum and Prorocentrum donghaiense,while in the exponential growth stage the release seemed relatively weak.During Ulva prolifera blooms,the released organic ligands increased the copper complexing capacity(CuCC)of the seawater in the enclosed seawater up to 2.82 times that of the external area.In addition,the field data indicated that the accumulation and degradation of Ulva prolifera increased CuCC by 37.81%in the green tide area.The increase in CuCC during algae bloom was attributed to the labile dissolved organic matter,such as protein-like substances directly secreted by algae,while at the end of the growth,it was attributed to the relatively inert byproducts such as humic substances.sublethal copper stress accelerated the U.prolifera degradation as well as the release of organic ligands,resulting in the CuCC 2.78 times higher than the control group.Meanwhile,the free Cu^(2+)in the system were effectively complexed,reducing to 6.27%of the initial level at the end of the cultivation,which significantly reduced the biological toxicity of Cu^(2+).

关 键 词：络合容量 海洋藻类 藻源有机质 重金属 伏安法 

分 类 号：P735[天文地球—海洋生物学]