鞭毛可视化方法及其在鞭毛功能研究中的应用  

Visualization of flagella and its applications in research on flagellar functions

作　　者：孔东洋 王璐 张红[3] 张静超 KONG Dongyang;WANG Lu;ZHANG Hong;ZHANG Jingchao(Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China;China Petroleum Corporation Science and Technology Research Institute Co.,Ltd.,Beijing 100083,China;Petrochemical Research Institute of PetroChina Co.,Ltd.,Beijing 102206,China;Medical Genetics Center,Sichuan Provincial People’s Hospital,University of Electronic Science and Technology of China,Chengdu 610054,Sichuan,China)

机构地区：[1]天津大学化工学院合成生物学前沿科学中心和系统生物工程教育部重点实验室,天津300350 [2]中国石油集团科学技术研究院有限公司,北京100083 [3]中国石油天然气股份有限公司石油化工研究院,北京102206 [4]电子科技大学四川省人民医院医学遗传中心,四川成都610054

出　　处：《生物工程学报》2025年第1期117-130,共14页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(2018YFA0902102);中国博士后科学基金(2023M740523);四川省人民医院博士后基金(2022B1013)。

摘　　要：鞭毛作为细菌主要的运动器官,是细菌表面重要的蛋白质结构,在细菌运动、趋化性、致病性以及环境感知等方面发挥着重要作用。随着细菌显微追踪技术的发展以及鞭毛可视化工具的开发应用,鞭毛新的运动形态和在细菌生理活动中更加详细的功能作用被逐步发现。本文主要综述了鞭毛可视化方法以及基于此方法对鞭毛功能的研究进展,为研究鞭毛功能提供了更多的思路,为未来其在抑制细菌传播和治疗细菌感染等领域的应用提供了理论基础。Flagella are important protein structures on the cell surface of bacteria and the main appendage for bacterial swimming.Flagella play a crucial role in bacterial motility,chemotaxis,pathogenicity,and environmental sensing.With the development of microscopic tracking technology and flagellum visualization tools,new forms of flagellar motility and increasing roles of flagella in the physiological activities of bacteria have been discovered.This review introduces the visualization methods of flagella and the applications of these methods in studying flagellar functions,giving insights into exploring the functions of flagella and laying a theoretical foundation for its future applications in inhibiting bacterial transmission and treating bacterial infections.

关 键 词：鞭毛 可视化 功能 运动性 致病性 

分 类 号：Q934[生物学—微生物学]