Effect of Fluorine Atoms and Piperazine Rings on Biotoxicity of Norfloxacin Analogues:Combined Experimental and Theoretical Study  

作　　者：Chuanxi Yang Xiaoning Wang Xinyan Zhao Yongkun Wu Jingyan Lin Yuhan Zhao Yiyong Xu Kaipeng Sun Chao Zhang Ziheng Wan Weihua Zhao Yihua Xiao Haofen Sun Dong Chen Wenping Dong Tieyu Wang Weiliang Wang 

机构地区：[1]School of Environmental and Municipal Engineering,Qingdao University of Technology,Qingdao 266520,China [2]Business School,Qingdao University of Technology,Qingdao 266520,China [3]Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention,Shantou University,Shantou 515063,China

出　　处：《Environment & Health》2024年第12期886-901,共16页环境与健康(英文)

摘　　要：To clarify the effect of the fluorine atom and piperazine ring on norfloxacin(NOR),NOR degradation products(NOR-DPs,P1−P8)were generated via UV combined with hydrogen peroxide(UV/H2O2)technology.NOR degradation did not significantly affect cytotoxicity of NOR against BV2,A549,HepG2,and Vero E6 cells.Compared with that of NOR,mutagenicity and median lethal concentration of P1−P8 in fathead minnow were increased,and bioaccumulation factor and oral median lethal dose of P1−P8 in rats were decreased.Molecular docking was used to evaluate the inhibitory effect of DNA gyrase A(gyrA)on NOR-DPs to determine the molecular-level mechanism and establish the structure−activity relationship.Results indicated that the most common amino acid residues were Ile13,Ser27,Val28,Gly31,Asp36,Arg46,Arg47,Asp157,and Gly340;hydrogen bonds and hydrophobic interactions played key roles in the inhibitory effect.Binding area(BA)decreased from 350.80Å2(NOR)to 346.21Å2(P1),and the absolute value of binding energy(|BE|)changed from 2.53 kcal/mol(NOR)to 2.54 kcal/mol(P1),indicating that the fluorine atom mainly affects BA.The piperazine ring clearly influenced BA and|BE|.“Yang ChuanXi Rules”were used to explain effects of molecular weight(MW),BA,|BE|,and sum ofη1+η2(η1:normalization of BA,η2:normalization of|BE|)and predict biotoxicity of NOR-DPs based on half-maximum inhibitory concentration(IC50),half-minimal inhibitory concentration(MIC50),and half-minimal bactericidal concentration(MBC50)values.

关 键 词：NORFLOXACIN biotoxicity inhibition effect molecular docking structure−activity relationship 

分 类 号：O62[理学—有机化学]