连花清瘟方剂对巴氏杆菌和猪丹毒丝菌的抗菌作用  

The antibacterial effects of Lianhua-Qingwen formula on Pasteurella multocida and Erysipelothrix rhusiopathiae

作　　者：刘晓瑜 王彤 周帮会 姜国均 朱伟峰 LIU Xiaoyu;WANG Tong;ZHOU Banghui;JIANG Guojun;ZHU Weifeng(College of Veterinary medicine/College of traditional chinese veterinary medicine,Hebei Agricultural University,Baoding 071000,China;Hebei Veterinary Biotechnology Innovation Center,Baoding 071000,China)

机构地区：[1]河北农业大学动物医学院/中兽医学院,河北保定071000 [2]河北省兽医生物技术创新中心,河北保定071000

出　　处：《养殖与饲料》2025年第2期51-55,共5页Animals Breeding and Feed

基　　金：河北省省属高等学校基本科研业务费项目(KY2023002);河北省自然科学基金项目(C2022204228);河北省现代农业产业技术体系建设专项(HBCT2024230201)。

摘　　要：[目的]分析中药连花清瘟方剂对2种常见畜禽病原菌(巴氏杆菌、猪丹毒丝菌)的抗菌作用,为其在兽医临床中的应用提供参考。[方法]首先,通过微量肉汤稀释法测定连花清瘟方剂对巴氏杆菌3株不同菌株(巴氏杆菌X7株、巴氏杆菌YU株、巴氏杆菌SD株)的最小杀菌浓度(MBC)和最小抑菌浓度(MIC)。然后,进行药物治疗试验。取4~6周龄小鼠50只,分为5组,巴氏杆菌攻毒后给予各组不同的治疗方案(连花清瘟方剂、无菌水、阿莫西林、头孢克洛)治疗3 d,通过治疗有效率评价连花清瘟方剂对巴氏杆菌感染的治疗效果;参照连花清瘟方剂抗巴氏杆菌的试验方法,测定连花清瘟方剂对猪丹毒丝菌的MIC、MBC和治疗有效率。[结果]连花清瘟方剂对巴氏杆菌不同菌株的MIC和MBC分别为4~8、8~16 mg/mL,对巴氏杆菌感染的治疗有效率为60%,显著高于对照组,与抗生素灌服组治疗无显著差异。连花清瘟方剂对猪丹毒丝菌SE38株的MIC值为64 mg/mL,MBC值为128mg/mL,小鼠感染模型中无显著治疗效果。[结论]连花清瘟方剂对巴氏杆菌具有较好的抗菌效果,可作为抗菌药替代品进一步研究。[Objectives]The antibacterial effects of traditional Chinese medicine Lianhua-Qingwen formula on two common pathogens in livestock and poultry including Pasteurella multocida and Erysipelothrix rhusio‐pathiae were studied to provide reference for its application in veterinary clinic.[Methods]The minimum bacte‐ricidal concentration(MBC)and minimum inhibitory concentration(MIC)of Lianhua-Qingwen formula against three different strains of Pasteurella including Pasteurella X7,Pasteurella YU,Pasteurella SD were measured with the microbroth dilution method.The experiment of drug therapy was conducted.50 mice aged 4-6 weeks were divided into 5 groups.Each group after infection with Pasteurella was treated with different therapeutic regimens including Lianhua-Qingwen formula,sterile water,amoxicillin,cefaclor for 3 days.The therapeutic effect of Lianhua-Qingw formula on Pasteurella infection was evaluated with the efficacy of treat‐ment.The MIC,MBC,and the efficacy of treatment of Lianhua-Qingwen formula against Erysipelothrix rhusio‐pathiae were determined according to the anti-Pasteurella test method of Lianhua-Qingwen formula.[Results]The MIC and MBC of Lianhua-Qingwen formula for different strains of Pasteurella was 4-8 mg/mL and 8-16 mg/mL,respectively.The efficacy of treatment for Pasteurella infection was 60%,significantly higher than that in the control group,and there was no significant difference in treatment compared to the antibiotic oral group.The MIC and MBC of Lianhua-Qingwen formula against SE38 strain of Erysipelothrix rhusiopathiae was 64 mg/mL and 128 mg/mL,and there was no significant therapeutic effect in the mouse infection model.[Conclu‐sions]Lianhua-Qingwen formula has a good antibacterial effect on Pasteurella,and can be can be further stud‐ied as a substitute for antibiotics.

关 键 词：连花清瘟方剂 巴氏杆菌 猪丹毒丝菌 减抗 替抗 治疗有效率 

分 类 号：S853.74[农业科学—临床兽医学]