大麻类纤维素合酶基因家族生物信息学分析  

Bioinformatics Analysis of Cellulose Synthase-like Gene Family in Cannabis sativa

作　　者：郭蓉[1] 吕品 张庆滢[1] 张园 陈璇[1] 许艳萍[1] 郭孟璧[1] 字雪靖 杨若菡[1] 杨明[1] GUO Rong;LYU Pin;ZHANG Qingying;ZHANG Yuan;CHEN Xuan;XU Yanping;GUO Mengbi;ZI Xuejing;YANG Ruohan;YANG Ming(Industrial Crops Research Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205,China)

机构地区：[1]云南省农业科学院经济作物研究所,云南昆明650205

出　　处：《贵州农业科学》2025年第1期1-9,共9页Guizhou Agricultural Sciences

基　　金：优势特色作物品种技术提升及转化利用专项;云南省农业基础研究联合专项面上项目(202301BD70001-126);国家麻类产业技术体系项目(CARS-16-E07)。

摘　　要：【目的】探明大麻类纤维素合酶(Cellulose synthase-like,Csl)基因家族成员的结构和功能,为调控大麻生长发育提供参考。【方法】通过生物信息学方法对大麻Csl家族成员的理化性质、系统进化、基因结构和保守基序及启动子顺式作用元件等进行分析。【结果】从大麻中共鉴定得到24个Csl家族成员,除7号染色体外其余9条染色体上均有成员分布,各成员外显子数目为3~9个。系统进化树分析显示,24个家族成员被分为CslA、CslB、CslC、CslD、CslE、CslG共6个亚族,其中CslC和CslD成员最多,均有6个。保守基序分析发现,Motif2和Motif3在6个亚族蛋白中均有分布,而Motif6、Motif8和Motif9仅在CslA和CslC亚族蛋白中分布,Motif1、Motif4、Motif7和Motif10仅在CslB、CslD、CslE和CslG亚族蛋白中分布。CsCsls基因启动子区域包含光响应、激素诱导元件、逆境响应元件和MYB结合位点。经公共转录组数据分析发现,除CsCslB2在叶中特异表达外,大部分成员在根和茎中表达水平明显高于叶片。不同亚族成员在茎秆不同组织和不同部位及下胚轴不同发育时期的表达量也存在一定差异。【结论】推测Csl家族基因除介导大麻纤维中半纤维素合成外,还参与大麻生长发育和逆境响应等其他生物学进程。【Objective】The structure and function of the cellulose synthase-like(Csl)family in Cannabis sativa were investigated to provide a reference for regulating the growth and development of C.sativa.【Method】The physicochemical properties,phylogenetic relationships,gene structures,conserved motifs and promoter cis-acting elements of the Csl family members in C.sativa were analyzed by the bioinformatics method.【Result】24 Csl family members were identified from C.sativa and these members were unevenly distributed on 9 chromosomes except chromosome 7.The exons number of these genes ranged from 3 to 9.These members were classified into six subfamilies(CslA,CslB,CslC,CslD,CslE,CslG)according to the phylogetic relationship.Both CslC and CslD had the largest subfamily members with 6 respectively.Motif2 and motif3 were observed in every subfamily,while motif6,motif8 and motif9 were observed only in CslA and CslC subfamily proteins and motif 1,motif4,motif7 and motif10 only in CslB,CslD,CslE and CslG subfamily proteins.The promoter region of CsCsls gene contained photo responsive,hormone inducing elements,stress responsive elements and MYB binding sites.Public transcriptomic data showed the expression levels of most members in roots and stems were higher than those in leaves except that CsCslB2 specially expressed in leaves.Different subfamily members had different expression levels in different tissues and parts of stems and hypocotyl at various developmental stages.【Conclusion】In addition to mediating the hemicelluloses synthesis in cellulose of C.sativa,the Csl family members might also participate in some other biological processes such as growth and development and environmental stress.

关 键 词：大麻 半纤维素 类纤维素合酶 基因家族 生物信息学 逆境响应 

分 类 号：S563.3[农业科学—作物学] Q943.2[生物学—植物学]