大孔树脂纯化蒙自藜芦总生物碱提取工艺研究  

Study on the Purification Technology of Total Alkaloids from the Veratrum mengtzeanum with Macroporous Resin

作　　者：吴倩 侯安国 朱常成 尹子丽 WU Qian;HOU Anguo;ZHU Changcheng;YIN Zili(Yunnan University of Chinese Medicine,Yunnan Dai Medicine and Key Laboratory of Yi Medicine,Kunming 650000,China;Yunnan University of Chinese Medicine,College of Traditional Chinese Medicine,Kunming 650000,China;Yunnan Free Trade Zone Ruizhicheng Pharmaceutical Technology Co.,Ltd.Kunming 650217,China)

机构地区：[1]云南中医药大学云南省傣医药与彝医药重点实验室,云南昆明650000 [2]云南中医药大学中药学院,云南昆明650000 [3]云南自由贸易试验区睿之成医药科技有限公司,云南昆明650217

出　　处：《中国民族民间医药》2025年第1期44-48,共5页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：云南省科技厅-云南中医药大学应用基础研究联合专项(202101AZ070001-322202101AZ070001-196,202301AZ070001-034);云南省教育厅科学研究基金项目(2021J0407,2023J1491);云南省傣医药与彝医药重点实验师室开放课题(202210ZD2208,202210SS2212)。

摘　　要：目的:对蒙自藜芦总生物碱提取工艺进行优化。方法:以蒙自藜芦总生物碱含量及3个成分藜芦碱、藜芦胺、介芬胺为指标,通过静态吸附和动态吸附实验考查上样体积、上样液质量浓度、径高比、上样液PH值、水洗用量、洗脱剂及其用量,优化出最佳纯化工艺条件。结果:选用D101型大孔树脂纯化蒙自藜芦总生物碱提取物,上样体积50 mL,径高比1∶5,上样液总生物碱质量浓度为0.2 mg/mL,上样液pH值为9,水洗用量为4 BV,洗脱剂为80%乙醇,洗脱剂用量为8 BV,纯化后的蒙自藜芦总生物碱转移率大于60%,含量为62.88%,比纯化前提高5.74倍。结论:D101型大孔树脂纯化蒙自藜芦总生物碱工艺稳定可行,可为后续制剂开发提供参考依据。Objective In Veratrum mengtzeanum total alkaloid extraction technology was optimized.Methods Taking the total al kaloid content and three components veratrine,veratrine,and mesofenamine as indicators,the sample volume,mass concentration of sample solution,diameter/height ratio,pH value of sample solution,amount of water washing,eluent and its amount of sample solu tion were examined by static adsorption and dynamic adsorption experiments,and the best purification process conditions were opti mized.Results D101 macroporous resin was used to purify the total alkaloid extract of Veratrum mengtzeanum.The sample volume was 50mL and the diameter/height ratio was 1∶5,on the quality of samples,total alkaloids concentration is 0.2 mg/mL,pH value of the sample fluid 9,water dosage of 4 bv,eluent is 80%ethanol,dosage of eluent is 8 bv,purified of Veratrum mengtzeanum transfer rate more than 60%of total alkaloid content is 62.88%,and 5.74 times higher than that of before purification.Conclusion D101 macro porous resin purification process is stable and feasible,which can provide a reference for the subsequent preparation development.

关 键 词：蒙自藜芦 总物碱 大孔树脂 纯化工艺 

分 类 号：R284.1[医药卫生—中药学]