黄腐酸缓解胡萝卜幼苗干旱胁迫机制研究  

Mechanism of Fulvic Acid Alleviating Drought Stress in Carrot Seedlings

作　　者：宋红霞[1] 宋甜月 申瑞雪 张洪涛 朱伟龙 高崇真 马思嘉 SONG Hongxia;SONG Tianyue;SHEN Ruixue;ZHANG Hongtao;ZHU Weilong;GAO Chongzhen;MA Sijia(College of Horticulture,Shanxi Agricultural University,Taigu,Shanxi 030801)

机构地区：[1]山西农业大学园艺学院,山西太谷030801

出　　处：《核农学报》2025年第3期641-654,I0009,共15页Journal of Nuclear Agricultural Sciences

基　　金：山西省重点研发计划重点项目(202102140601013);山西省现代农业产业技术体系建设专项资金项目(2024CYJSTX08)。

摘　　要：胡萝卜苗期对水分需求量较大,在干旱胁迫下易出现缺苗、死苗现象,最终导致产量下降。为明确黄腐酸缓解胡萝卜幼苗干旱胁迫的作用,本研究以甜红1号胡萝卜为试验材料,叶面喷施不同浓度黄腐酸,基于转录组测序技术并结合生理分析解析黄腐酸缓解胡萝卜幼苗干旱胁迫的生理和分子机制。结果表明,黄腐酸处理可以有效缓解干旱胁迫对胡萝卜幼苗生长的抑制,降低相对电导率(REC)及丙二醛(MDA)、过氧化氢(H_(2)O_(2))和超氧阴离子(O_(2)^(-))含量,提高抗氧化酶活性及可溶性糖、脯氨酸含量和光合相关参数,并显著降低脱落酸(ABA)含量,最佳黄腐酸浓度为800 mg·L^(-1)。干旱+喷施0 mg·L^(-1)黄腐酸和干旱+喷施800 mg·L^(-1)黄腐酸处理组转录组分析共获得541个差异表达基因(DEGs),筛选出与抗旱相关的关键差异表达基因11个,其中3个渗透调节相关基因、3个抗氧化酶相关基因、2个光合相关基因、3个植物激素信号传导相关基因,且上述基因的表达模式与测定的相应生理指标变化规律基本一致。综合分析得出,黄腐酸通过调控淀粉和蔗糖代谢、氧化还原酶活性以及植物激素信号转导等通路协同缓解胡萝卜幼苗干旱胁迫。本研究结果为黄腐酸在缓解胡萝卜苗期干旱胁迫中的应用提供了理论支撑。Carrot seedlings require a high amount of water,and they are prone to insufficiency and death,ultimately resulting in a decline in yield under drought stress.In order to clarify the effect of fulvic acid(FA)on alleviating drought stress of carrot seedlings,carrot Tianhong No.1 was used as experimental material,and was sprayed different concentrations of fulvic acid on the leaf surface,the physiological and molecular mechanism of FA alleviating drought stress in carrot seedlings were analyzed based on transcriptome sequencing technology and physiological analysis.The results showed that FA could effectively alleviate the inhibition of drought stress on the growth of carrot seedlings,reduce the relative conductivity(REC)and contents of malondialdehyde(MDA),hydrogen peroxide(H_(2)O_(2))and superoxide anion(O_(2)^(−)),increase the activity of antioxidant enzymes,soluble sugar,proline contents and photosynthetic parameters,and significantly reduce the content of abscisic acid(ABA).The optimal FA concentration was 800 mg·L^(-1).A total of 541 differentially expressed genes were obtained by transcriptome analysis of drought without fulvic acid and drought with spraying 800 mg·L^(-1) fulvic acid,in which 11 key DEGs related to drought resistance were screened out,including 3 osmoregulation DEGs,3 antioxidase-related DEGs,2 photosynthesis DEGs and 3 plant hormone signaling DEGs,and the expression patterns of these genes were basically consistent with the corresponding physiological indexes.It was concluded that FA could synergically alleviate drought stress in carrot seedlings by regulating starch and sucrose metabolism,oxidoreductase enzyme activity and plant hormone signal transduction.The results of this study provide theoretical support for the application of fulvic acid in alleviating drought stress during carrot seedling stage.

关 键 词：黄腐酸 胡萝卜 干旱胁迫 抗旱生理 转录组 

分 类 号：S631.2[农业科学—蔬菜学]