Endogenous retroviral ERVH48‑1 promotes human urine cell reprogramming  

作　　者：Yuling Peng Jieying Zhu Qi Zhang Ran Zhang Zhenhua Wang Zesen Ye Ning Ma Dajiang Qin Duanqing Pei Dongwei Li 

机构地区：[1]Key Laboratory of Biological Targeting Diagnosis,Therapy and Rehabilitation of Guangdong Higher Education Institutes,The Fifth Affiliated Hospital of Guangzhou Medical University,Guangzhou 510799,China [2]Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory),Guangzhou 510005,China [3]CAS Key Laboratory of Regenerative Biology,Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine,Institutes of Biomedicine and Health,GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre,Hong Kong Institute of Science&Innovation,Guangzhou,Guangzhou,Guangdong 510530,China [4]State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macao,Macao,China [5]Guangzhou National Laboratory,Guangzhou,China [6]Laboratory of Cell Fate Control,School of Life Sciences,Westlake University,Hangzhou 310024,China [7]GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis,Therapy and Rehabilitation,The Fifth Affiliated Hospital,Guangzhou Medical University,Guangzhou,China [8]Guangdong Engineering Research Center of Early Clinical Trials of Biotechnology Drugs,The Fifth Affiliated Hospital,Guangzhou Medical University,Guangzhou,China

出　　处：《Cell Regeneration》2024年第1期212-226,共15页细胞再生(英文)

基　　金：National Natural Science Foundation of China(32270574,92068201);Science and Technology Projects in Guangzhou(2023A03J0045);Guangzhou Key Laboratory of Biological Targeting Diagnosis and Therapy(202201020379);Key Laboratory of Guangdong Higher Education Institutes(2021KSYS009).

摘　　要：Endogenous retroviruses(ERVs),once thought to be mere remnants of ancient viral integrations in the mammalian genome,are now recognized for their critical roles in various physiological processes,including embryonic devel-opment,innate immunity,and tumorigenesis.Their impact on host organisms is significant driver of evolutionary changes,offering insight into evolutionary mechanisms.In our study,we explored the functionality of ERVs by exam-ining single-cell transcriptomic profiles from human embryonic stem cells and urine cells.This led to the discovery of a unique ERVH48-1 expression pattern between these cell types.Additionally,somatic cell reprogramming efficacy was enhanced when ERVH48-1 was overexpressed in a urine cell-reprogramming system.Induced pluripotent stem cells(iPSCs)generated with ERVH48-1 overexpression recapitulated the traits of those produced by traditional repro-gramming approaches,and the resulting iPSCs demonstrated the capability to differentiate into all three germ layers in vitro.Our research elucidated the role of ERVs in somatic cell reprogramming.

关 键 词：Endogenous retroviruses ERVH48-1 Human embryonic stem cells Induced pluripotent stem cells Urine cell integration-free reprogramming system 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]