Asymmetric porous composite hydrogel patch for microenvironment-adapted repair of contaminated abdominal wall defects  

作　　者：Yang Yu Yinxiang Tang Weiwen Liang Yuanbin Wang Yang Ouyang Wenxuan Xiong Bingna Zheng Lili Chu Hui Wang 

机构地区：[1]Department of General Surgery(Colorectal Surgery),Guangdong Institute of Gastroenterology,Biomedical Innovation Center,Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases,The Sixth Affiliated Hospital,Sun Yat-sen University,Guangzhou 510655,PR China [2]PCFM Lab,School of Chemistry,Sun Yat-sen University,Guangzhou 510006,PR China [3]Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou 510006,PR China [4]The Eighth Affiliated Hospital,Sun Yat-sen University,Shenzhen 518000,PR China

出　　处：《Engineered Regeneration》2024年第4期468-481,共14页再生工程(英文)

基　　金：supported by the projects of the National Key R&D Program of China(2021YFC2101700);National Natural Science Foundation of China(52372057,22371312);Guangdong Major Project of Basic and Applied Basic Research(2023B0303000025);Sun Yat-sen University Clinical Research 5010 Program(2017008);Medical Scientific and Technical Foundation of Guangdong Province(A2023085);Fundamental Research Funds for the Central Universities,Sun Yat-sen University(23yxqntd002);Guangdong Basic Research Center of Excellence for Functional Molecular Engineering.

摘　　要：Effective antibacterial property and long-term mechanical support are essential for the repair of complex ab-dominal wall defects associated with infection.However,clinically available repair materials often fail to meet these requirements,resulting in high surgical failure rate and complications.In this study,an asymmetric porous composite hydrogel patch(cCS/PVA@BAC)with antibacterial,anti-adhesion,pro-healing,and durable mechan-ical support properties is designed for the efficient repair of contaminated abdominal wall defects.By stepwise phase-conversion and soaking method,robust and stable polyvinyl alcohol hydrogel(PVAH)is integrated with the biocompatible multicomponent hydrogel made of chitosan and carboxymethyl chitosan(cCS),and benza-lkonium chloride(BAC)is loaded to enhance the antibacterial property.The cCS layer of cCS/PVA@BAC has an extracellular matrix-like porous structure,which can promote fibroblasts adhesion and wound healing.In contrast,the PVAH layer on the other side with a smooth and dense structure,which can reduce fibroblasts adhesion and prevent visceral adhesion.In addition,the composite hydrogel patch has good anti-swelling and anti-deformation properties as well as stable mechanical strength,thus can withstand high intraperitoneal pres-sure in the wet internal microenvironment.The loaded BAC can efficiently kill bacteria and improve the local inflammatory microenvironment.With these advantages,cCS/PVA@BAC can significantly reduce inflammation,promote tissue remodeling,and accelerate the healing of contaminated abdominal wall defects in the rat model.These findings suggest a potential use of multifunctional hydrogel patch as an ideal material for effective repair of contaminated soft tissue defects.

关 键 词：Contaminated abdominal wall defects Asymmetric porous composite hydrogel CHITOSAN Polyvinyl alcohol Microenvironment-adapted repair 

分 类 号：TB3[一般工业技术—材料科学与工程]