七氟烷对结核分枝杆菌体外抑菌效果的评价  

Evaluation of the in vitro antimicrobial effects of sevoflurane on Mycobacterium tuberculosis

作　　者：张超 于霞 黄海荣 刘伟[2] 刘涛[2] Zhang Chao;Yu Xia;Huang Hairong;Liu Wei;Liu Tao(National Clinical Laboratory on Tuberculosis,Beijing Key Laboratory for Drug Resistant Tuberculosis Research,Beijing Chest Hospital,Capital Medical University,Beijing Tuberculosis and Thoracic Tumor Institute,Beijing 101149,China;Department of Anesthesiology,Beijing Chest Hospital,Capital Medical University,Beijing 101149,China)

机构地区：[1]首都医科大学附属北京胸科医院/国家结核病临床实验室,北京101149 [2]首都医科大学附属北京胸科医院麻醉科,北京101149

出　　处：《中国防痨杂志》2025年第2期158-163,共6页Chinese Journal of Antituberculosis

摘　　要：目的:探究临床常用镇静、镇痛和肌肉松弛剂对结核分枝杆菌(Mycobacterium tuberculosis,MTB)的体外抑菌活性。方法:检测5种临床常用镇静剂(包括吸入用的七氟烷和静脉用的丙泊酚)、镇痛剂地佐辛和肌肉松弛剂(罗库溴铵和阿曲库铵)对MTB标准株H37Rv的最低抑菌浓度(minimal inhibit concentration,MIC)。对具有较好体外抑菌活性的七氟烷通过菌落形成单位(CFU)计数方法观察不同浓度处理后MTB的菌落数量变化。制备痰菌悬液模拟痰液环境,观察七氟烷在4 h内的抗结核活性;使用人单核细胞白血病细胞(THP-1)验证4 h内七氟烷的细胞毒性。结果:丙泊酚、地佐辛、罗库溴铵和阿曲库铵对MTB均无明显的体外抑菌活性,仅七氟烷在体外对H37Rv有抑菌效果,MIC值为1.25%。进一步发现,3%、6%、9%浓度七氟烷处理8 h MTB的菌落计数(log10CFU)分别为3.086±0.196、4.165±0.083和4.162±0.100,均明显低于对照组(4.489±0.083),差异有统计学意义(F=7.630,P=0.003);9%七氟烷处理48 h MTB的菌落计数(log10CFU)为3.000±0.000,明显低于对照组的5.077±0.191,差异有统计学意义(F=313.300,P<0.001)。在痰菌悬液中,用9%七氟烷处理2 h和4 h可产生明显的抗MTB活性,MTB菌落计数(log10CFU)分别为5.846±0.384和5.947±0.280,均明显低于对照组[MTB菌落计数(log10CFU)分别为6.264±0.150和6.331±0.162],差异均有统计学意义(F值分别为4.173和6.091,P值分别为0.025和0.006)。并且CCK-8实验发现,0~9%浓度七氟烷处理THP-1细胞4 h内均可维持细胞存活率在(81.033±3.190)%以上。结论:七氟烷具有良好的体外抗MTB活性,在模拟痰液环境中保持短时间杀菌的效果,且安全性良好。Objective:To investigate the in vitro antimicrobial activities of commonly used sedatives,analgesics,and muscle relaxants against Mycobacterium tuberculosis(MTB).Methods:The minimum inhibitory concentrations(MICs)of five clinically common sedatives,including sevoflurane and propofol,the analgesic dezocine,and the muscle relaxants rocuronium and atracurium,were determined against the standard MTB strain H37Rv.Colony-forming unit(CFU)counting was employed to evaluate the changes in MTB colony numbers following treatment with varying concentrations of sevoflurane,which demonstrated notable antimicrobial activity in vitro.A sputum-bacterial suspension was prepared to simulate the sputum environment,and the anti-tuberculosis activity of sevoflurane was evaluated over a 4-hour period.Additionally,the cytotoxicity of sevoflurane within 4 hours was assessed using the THP-1 cell line.Results:Propofol,dexmedetomidine,rocuronium bromide,and atracurium demonstrated no significant in vitro antibacterial activity against MTB.In contrast,sevoflurane exhibited potent antibacterial activity against H37Rv in vitro,with a MIC value of 1.25%.Further analysis showed that treatment with sevoflurane at concentrations of 3%,6%,and 9%for 8 hours reduced MTB colony counts(log10CFU)to 3.086±0.196,4.165±0.083,and 4.162±0.100,respectively.These values were significantly lower than those of the control group(4.489±0.083),with statistical significance(F=7.630,P=0.003).The colony count(log10CFU)of MTB treated with 9%sevoflurane for 48 hours was 3.000±0.000,significantly lower than that of the control group(5.077±0.191),with a statistically significant difference(F=313.300,P<0.001).In sputum suspension,treatment with 9%sevoflurane for 2 and 4 hours demonstrated significant anti-MTB activity,reducing MTB colony counts(log10CFU)to 5.846±0.384 and 5.947±0.280,respectively.These values were significantly lower than those in the control group(log10CFU:6.264±0.150 and 6.331±0.162,respectively),with statistically significant differences(F=4.1

关 键 词：分枝杆菌 结核 微生物敏感性试验 催眠药和镇静药 体外研究 七氟烷 

分 类 号：R378.91[医药卫生—病原生物学] R917[医药卫生—基础医学]