高通量测序技术在骨关节结核诊疗中的研究进展  

Research progress of high-throughput sequencing technology in the diagnosis and treatment of osteoarticular tuberculosis

作　　者：徐子安 浦飞飞[2] 冯晶[2] 夏平 Xu Zian;Pu Feifei;Feng Jing;Xia Ping(Department of Osteology,The Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430014,China;Department of Osteology,Wuhan Hospital of Traditional Chinese and Western Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Osteology,Wuhan Fourth Hospital,Wuhan 430033,China)

机构地区：[1]华中科技大学同济医学院附属武汉市中心医院骨科,武汉430014 [2]华中科技大学同济医学院附属武汉市中西医结合医院骨科,武汉430022 [3]武汉市第四医院骨科,武汉430033

出　　处：《中国防痨杂志》2025年第2期224-230,共7页Chinese Journal of Antituberculosis

基　　金：武汉市卫生健康委西医药类重大项目(WX21M02)。

摘　　要：骨关节结核约占肺外结核的10%,传统的诊断方法如分枝杆菌培养和免疫学检测存在敏感度低、误诊率和漏诊率高等问题。GeneXpert MTB/RIF在骨关节结核的诊断中具有重要作用,但存在无法检测其他病原菌、肺外结核检测敏感度有限等不足。高通量测序技术不仅提供了快速和精确的病原体检测方法,还在骨关节结核治疗指导和耐药性检测方面显示出巨大的潜力。作者就高通量测序技术在骨关节结核中的应用进行综述,以期为该疾病的诊断和治疗提供参考。Osteoarticular tuberculosis constitutes approximately 10%of all extrapulmonary tuberculosis cases.Traditional diagnostic methods,such as mycobacterial culture and immunological assays,are hindered by low sensitivity and high rates of misdiagnosis and missed diagnosis.The GeneXpert MTB/RIF molecular diagnostic technology has been pivotal in diagnosing osteoarticular tuberculosis;however,it has notable limitations,including an inability to detect non-tuberculous pathogens and reduced sensitivity in extrapulmonary samples.In contrast,high-throughput sequencing(HTS)technology provides a rapid,highly accurate method for pathogen detection,and it holds substantial promise for guiding treatment strategies and identifying drug resistance in osteoarticular tuberculosis.The review aims to explore the advancements in HTS technology applications in diagnosing and managing osteoarticular tuberculosis,offering new perspectives to improve disease control.

关 键 词：结核 骨关节 高通量测序技术 药物耐受性 

分 类 号：R37[医药卫生—病原生物学] R529.2[医药卫生—基础医学]