气滞血瘀型小鼠肝癌皮下移植瘤模型的建立与探索  

Establishment and exploration of a subcutaneous transplanted tumor model of hepatocellular carcinoma in mice with Qi stagnation and blood stasis syndrome

作　　者：曹琳娜 李铭哲 施展豪 王香香 潘志强[1] 彭佩克 CAOLinna;LIMingzhe;SHIZhanhao;WANGXiangxiang;PANZhiqiang;PENGPeike(School of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学中医学院,上海201203

出　　处：《中国实验动物学报》2024年第12期1505-1514,共10页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(82004000)。

摘　　要：目的探索气滞血瘀型小鼠肝癌皮下移植瘤模型的建立方法。方法通过气滞血瘀证候造模(7 d)联合肝癌皮下移植瘤造模(20 d),将40只C57BL/6雄性小鼠随机分为4组:空白组、气滞血瘀组、移植瘤组和复合模型组,观察造模后小鼠的证候表现以及肿瘤大小与重量。结果(1)体重变化:造模第7天,气滞血瘀组和复合模型组体重较空白组显著降低(P<0.05)。(2)体温变化:造模第7天,与空白组相比,气滞血瘀组体温显著降低(P<0.05),移植瘤组体温升高(P<0.05);造模第27天,复合模型组体温较空白组明显降低(P<0.05)。(3)证候表现:根据证候评分表进行打分,造模第7天,气滞血瘀组和复合模型组小鼠均出现气滞血瘀证候(P<0.05)。随着造模时间的延长,移植瘤组小鼠的评分随肿瘤的成型而上升;复合模型组的评分较其余3组均显著上升(P<0.05)。(4)爪部瘀点:与空白组相比,3组造模小鼠的爪部瘀点均明显增多(P<0.05),且复合模型组爪部瘀点最多。(5)爪部r值:与空白组相比,3组造模小鼠爪部r值均显著降低(P<0.05),且复合模型组爪部r值始终低于其余3组。(6)旷场活动度:与空白组相比,复合模型组小鼠的垂直和水平旷场活动度显著降低(P<0.05)。(7)凝血4项指标:与空白组相比,复合模型组活化部分凝血活酶时间(activated partial thromboplastin time,APTT)缩短、凝血酶时间(thrombin time,TT)缩短、纤维蛋白原(fibrinogen,FIB)明显增多(P<0.05或P<0.01)。(8)肿瘤大小与重量:与移植瘤组相比,复合模型组肿瘤大小和瘤重显著增加(P<0.05)。结论本研究成功构建了气滞血瘀型小鼠肝癌皮下移植瘤模型,表明肝癌病程中会出现气滞血瘀证候;此外,诱发气滞血瘀证候的病因,会进一步促进肝癌的进展。ObjectiveTo explore the establishment of a subcutaneously transplanted tumor model of hepatocellular carcinoma in mice with Qi stagnation and blood stasis syndrome.MethodsForty male C57BL/6 mice were randomly divided into 4 groups:NC group,QZXY group,Tumor group,and QZXY+Tumor group.They were categorized based on the modeling of Qi stagnation and blood stasis syndrome(7 days)combined with the modeling of subcutaneous transplantation of hepatocellular carcinoma tumor(20 days).Observations were conducted of the syndrome manifestations as well as the tumor size and weight of the mice after modeling.Results(1)Body weight:on the 7th day of modeling,the weights of the QZXY group and QZXY+Tumor group were significantly lower than that of the NC group(P<0.05).(2)Body temperature:on the 7th day of modeling,body temperature significantly decreased in the QZXY group(P<0.05),while it increased in the Tumor group(P<0.05)compared with the NC group.On the 27th day of modeling,the temperature of the QZXY+Tumor group was significantly lower than that of the NC group(P<0.05).(3)Syndrome manifestations:according to the syndrome scoring table,mice in both the QZXY group and QZXY+Tumor group exhibited Qi stagnation and blood stasis syndrome on the 7th day of modeling(P<0.05).As modeling time extended,the score of mice in the Tumor group increased with the formation of the tumor,and the score of mice in the QZXY+Tumor group was significantly higher than that of the other three groups(P<0.05).(4)Claw petechiae:the number of claw petechiae significantly increased in all three groups of modeled mice compared with the NC group(P<0.05),with the QZXY+Tumor group showing the highest number.(5)Claw r value:the r value of the claw was significantly lower in all three groups of modeled mice than that in the NC group(P<0.05).Additionally,the r value of the claw in the QZXY+Tumor group was consistently lower than that of the other three groups.(6)Open field activity:the vertical and horizontal activity of mice in the QZXY+Tumor group decreased si

关 键 词：肝癌 气滞血瘀 证候模型 病-证结合 

分 类 号：Q95-33[生物学—动物学]