基于过敏进程的“特应性皮炎-哮喘”小鼠模型的建立与优化  

Establishment and optimization of an“atopic dermatitis-asthma”mouse model based on allergic march

作　　者：任梦娇 严怡婷 张瑜[1] 瞿旻晔 刘涛[1] REN Mengjiao;YAN Yiting;ZHANG Yu;QU Minye;LIU Tao(School of Chinese Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学中医学院,南京210023

出　　处：《中国实验动物学报》2024年第12期1572-1580,共9页Acta Laboratorium Animalis Scientia Sinica

基　　金：江苏省自然科学基金(BK20230449);江苏省中医药科技发展计划青年基金项目(QN202010);国家中医药管理局高水平中医药重点学科建设项目资助(国中医药人教函[2023]85号)。

摘　　要：目的比较卵清蛋白(ovalbumins,OVA)及钙泊三醇(calcipotriol,MC903)单独或联合造模条件下模型小鼠皮肤及肺组织敏化进展情况,建立并优化“特应性皮炎-哮喘”过敏进程(atopic march,AM)动物模型。方法6~8周龄SPF级BALB/c小鼠40只,随机分为空白组、模型A组、模型B组和模型C组。三组模型小鼠分别以MC903、MC903+OVA和OVA为造模药物,通过2次皮肤敏化激发和1次呼吸道敏化激发建立AM模型。运用皮肤敏化严重程度评分系统、免疫组化和酶联吸附试验等方法,进行皮损形貌学比较、皮肤和肺组织病理学及免疫表型比较。结果与OVA或MC903单独造模相比较,MC903联合OVA构建的AM小鼠模型皮损形貌学改变更为显著、小鼠皮肤敏化严重程度评分更高、皮肤及气道炎症细胞浸润更为严重、相关炎症因子胸腺基质淋巴细胞生成素(thymic stromal lymphopoietin,TSLP)、白介素4(interleukin 4,IL-4)、白介素-13(interleukin 13,IL-13)及白介素-10(interleukin 10,IL-10)的表达变化更为显著(P<0.05)。结论运用MC903联合OVA优化的AM动物模型构建成功,为AM机制研究提供了良好的方法学基础。ObjectiveTo establish and optimize an animal model of atopic march(AM),skin and lung tissue sensitization under single or combined modeling of ovalbumins(OVA)and calcipotriol(MC903)was compared.Methods 40 SPF BALB/c mice aged 6~8 weeks were randomly divided into a control group,model group A,model group B and model group C.The 3 of AM models were established with MC903,MC903+OVA and OVA,respectively,through skin sensitization(twice)and respiratory sensitization(once).Skin sensitization severity scoring system,immunohistochemistry,and enzyme-linked adsorption assay were used to compare skin lesion morphology,skin or lung histopathology,and immunophenotype.ResultsCompared to OVA or MC903 modeling alone,MC903+OVA modeled mice showed more significant changes in skin morphology,a higher score for skin sensitization severity,more severe skin and airway inflammatory cell infiltration,and more significant changes in the expression of the related inflammatory factors thymic stromal lymphopoietin(TSLP),interleukin 4(IL-4),IL-13 and IL-10(P<0.05).ConclusionsAn AM animal model optimized by MC903 combined with OVA was successfully constructed that provides a good method ological basis for AM mechanism research.

关 键 词：特应性皮炎 哮喘 过敏进程 动物模型 

分 类 号：Q95-33[生物学—动物学]