Transcriptomic dynamics of ABA response in Brassica napus guard cells  

作　　者：Florent Villiers Yasir Suhail Jade Lee Felix Hauser Jaeung Hwang Joel S.Bader John K.McKay Scott C.Peck Julian I.Schroeder June M.Kwak 

机构地区：[1]Centre de Recherche de La Dargoire,Bayer CropScience,69009 Lyon,France [2]Department of Biomedical Engineering,Johns Hopkins University,Baltimore,MD 21205,USA [3]Department of Cell Biology and Molecular Genetics,University of Maryland,College Park,MD 20742,USA [4]Division of Biological Sciences,Cell and Developmental Biology Department,University of California San Diego,La Jolla,CA 92093,USA [5]Department of New Biology,DGIST,Daegu 42988,Republic of Korea [6]Department of Bioagricultural Sciences,Colorado State University,Fort Collins,CO 80523-1177,USA [7]Department of Biochemistry and Christopher S.Bond Life Sciences Center,Interdisciplinary Plant Group,University of Missouri,Columbia,MO 65211,USA

出　　处：《Stress Biology》2024年第1期139-155,共17页逆境生物学(英文)

基　　金：supported by a grant from National Science Foundation(IOS-1025837)to JMK;Sarah Assmann,JB,JKM,SCP,and JIS,and in part by grants from National Research Foundation(2020R1A4A1019408);Samsung Science and Technology Foundation(SSTF-BA2101-10)to JMK and from National Institutes of Health(GM060396)to JIS.

摘　　要：Drought has a significant,negative impact on crop production;and these effects are poised to increase with climate change.Plants acclimate to drought and water stress through diverse physiological responses,primarily mediated by the hormone abscisic acid(ABA).Because plants lose the majority of their water through stomatal pores on aerial surfaces of plants,stomatal closure is one of the rapid responses mediated by ABA to reduce transpirational water loss.The dynamic changes in the transcriptome of stomatal guard cells in response to ABA have been investigated in the model plant Arabidopsis thaliana.However,guard cell transcriptomes have not been analyzed in agronomically valuable crops such as a major oilseed crop,rapeseed.In this study,we investigated the dynamics of ABA-regulated transcriptomes in stomatal guard cells of Brassica napus and conducted comparison analysis with the transcriptomes of A.thaliana.We discovered changes in gene expression indicating alterations in a host of physiological processes,including stomatal movement,metabolic reprogramming,and light responses.Our results suggest the existence of both immediate and delayed responses to ABA in Brassica guard cells.Furthermore,the transcription factors and regulatory networks mediating these responses are compared to those identified in Arabidopsis.Our results imply the continuing evolution of ABA responses in Brassica since its divergence from a common ancestor,involving both protein-coding and non-coding nucleotide sequences.Together,our results will provide a basis for developing strategies for molecular manipulation of drought tolerance in crop plants.

关 键 词：Abscisic acid Guard cells RAPESEED Stomatal movement TRANSCRIPTOME 

分 类 号：Q94[生物学—植物学]