Deciphering the roles of bacterial and fungal communities in the formation and quality of agarwood  

作　　者：Chen-Chen Fu Bao-Xing Huang Shan-Shan Wang Yu-Chen Song Dolkar Metok Yu-Xiang Tan Tai-Ping Fan Alisdair RFernie Meisam Zargar Yan Wang Mo-Xian Chen Liang-Wen Yu Fu-Yuan Zhu 

机构地区：[1]State Key Laboratory of Tree Genetics and Breeding,the Southern Modern Forestry Collaborative Innovation Center,Key Laboratory of State Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation,College of Life Sciences,Nanjing Forestry University,Nanjing 210037,China [2]Clinical Laboratory,Shenzhen Children’s Hospital,Shenzhen 518000,China [3]Dongguan Institute of Guangzhou University of Chinese Medicine,Dongguan Institute of Guangzhou University of Chinese Medicine,Dongguan 523808,China [4]Guangdong Province Key Laboratory for Biotechnology Drug Candidates,Institute of Basic Medical Sciences and Department of Biotechnology,School of Life Sciences and Biopharmaceutics,Guangdong Pharmaceutical University,Guangzhou 510006,China [5]CAS Key Laboratory of Quantitative Engineering Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China [6]Department of Pharmacology,University of Cambridge,Cambridge,UK [7]School of Health Sciences,Fuyao University of Science&Technology,Fuzhou 350000,China [8]Max Planck Institute of Molecular Plant Physiology,Am Muhlenberg 1,14476 Potsdam-Golm,Germany [9]Department of Agrobiotechnology,Institute of Agriculture,RUDN University,117198 Moscow,Russia [10]Science and Technology Innovation Center,Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510405,China [11]State Key Laboratory of Desert and Oasis Ecology,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China.

出　　处：《Stress Biology》2024年第1期187-204,共18页逆境生物学(英文)

基　　金：supported by STI 2030-Major Projects(2023ZD040562);the Program for Science Technology and Innovation Committee of Shenzhen(No.2021N062-JCYJ20210324115408023);Administration of Traditional Chinese Medicine of Guangdong Province(No.20221218);the RUDN university Scientific Projects Grand System(No.040416-2-000);Guangdong Highlevel Hospital Construction Fund,Shenzhen High-level Hospital Construction Fund and Jiangsu Agricultural Science and Technology Innovation Fund(No.CX(21)2023);Natural Science Foundation of Guangdong Province(No.2021B1515140009).

摘　　要：Aquilaria sinensis is a significant resin-producing plant worldwide that is crucial for agarwood production.Agarwood has different qualities depending on the method with which it is formed,and the microbial community structures that are present during these methods are also diverse.Furthermore,the microbial communities of plants play crucial roles in determining their health and productivity.While previous studies have investigated the impact of microorganisms on agarwood formation,they lack comprehensiveness,particularly regarding the properties of the microbial community throughout the entire process from seedling to adult to incense formation.We collected roots,stems,leaves,flowers,fruits and other tissues from seedlings,healthy plants and agarwood-producing plants to address this gap and assess the dominant bacterial species in the microbial community structures of A.sinensis at different growth stages and their impacts on growth and agarwood formation.The bacteria and fungi in these tissues were classified and counted from different perspectives.The samples were sequenced using the Illumina sequencing platform,and sequence analyses and species annotations were performed using a range of bioinformatics tools to assess the plant community compositions.An additional comparison of the samples was conducted using diversity analyses to assess their differences.This research revealed that Listeria,Kurtzmanomyces,Ascotaiwania,Acinetobacter,Sphingobium,Fonsecaea,Acrocalymma,Allorhizobium,Bacillus,Pseudomonas,Peethambara,and Debaryomyces are potentially associated with the formation of agarwood.Overall,the data provided in this article help us understand the important roles played by bacteria and fungi in the growth and agarwood formation process of A.sinensis,will support the theoretical basis for the large-scale cultivation of A.sinensis,and provide a basis for further research on microbial community applications in agarwood production and beyond.

关 键 词：Aquilaria sinensis ENDOPHYTIC Exophytic FUNGI BACTERIA Plant tissue 

分 类 号：Q94[生物学—植物学]