Alginate oligosaccharide supplementation improves boar semen quality under heat stress  

作　　者：Yexun Zhou Zeou Wei Jiajian Tan Haiqing Sun Haidi Jiang Yang Gao Hongfu Zhang Martine Schroyen 

机构地区：[1]State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China [2]Precision Livestock and Nutrition Unit,Gembloux Agro-Bio Tech,University of Liege,Gembloux,Belgium [3]College of Life Science,Baicheng Normal University,Baicheng 137000,Jilin,China [4]YangXiang Joint Stock Company,Guigang 53700,China [5]School of Agriculture and Food Science,University College Dublin,Belfeld Dublin 4,Ireland

出　　处：《Stress Biology》2024年第1期243-257,共15页逆境生物学(英文)

基　　金：supported by the funding of CAAS of Agricultural Science and Technology Innovation Program(CAAS-ZDRW202006-02,ASTIPIAS07);the State Key Laboratory of Animal Nutrition(2004DA125184G2102).

摘　　要：Heat stress is a serious problem that affects animal husbandry by reducing growth and reproductive performance of animals.Adding plant extracts to the diet is an effective way to help overcome this problem.Alginate oligosaccharide(AOS)is a natural non-toxic antioxidant with multiple biological activities.This study analyzed the potential mechanism of AOS in alleviating heat stress and improving semen quality in boars through a combination of multiple omics tools.The results indicated that AOS could significantly increase sperm motility(P<0.001)and sperm concentration(P<0.05).At the same time,AOS improved the antioxidant capacity of blood and semen,and increased blood testosterone(P<0.05)level.AOS could improve the metabolites in sperm,change the composition of gut microbiota,increase the relative abundance of beneficial bacteria such as Pseudomonas(P<0.01),Escherichia-Shigella(P<0.05),Bifidobacterium(P<0.01),reduce the relative abundance of harmful bacteria such as Prevotella_9(P<0.05),Prevotellaceae_UCG-001(P<0.01),and increase the content of short chain fatty acids.Proteomic results showed that AOS increased proteins related to spermatogenesis,while decreasing heat shock protein 70(P<0.05)and heat shock protein 90(P<0.01).These results were verified using immunofluorescence staining technology.There was a good correlation among sperm quality,sperm metabolome,sperm proteome,and gut microbiota.In conclusion,AOS can be used as a feed additive to increase the semen quality of boars to enhance reproductive performance under heat stress.

关 键 词：Heat stress Alginate oligosaccharides(AOS) Semen quality Gut microbiota Sperm metabolome Sperm proteome BOARS 

分 类 号：R69[医药卫生—泌尿科学]