Identification of nuclear membrane SUN proteins and components associated with wheat fungal stress responses  

作　　者：Huan Guo Jianfeng Wang Di Yao Ligang Yu Wenting Jiang Lincai Xie Shikai Lv Xiangyu Zhang Yajuan Wang Changyou Wang Wanquan Ji Hong Zhang 

机构地区：[1]State Key Laboratory for Crop Stress Resistance and High-Efficiency Production,College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China [2]State Key Laboratory for Crop Stress Resistance and High-Efficiency Production,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi 712100,China [3]Engineering Research Center of Wheat Breeding,Ministry of Education,Yangling,Shaanxi 712100,China

出　　处：《Stress Biology》2024年第1期377-392,共16页逆境生物学(英文)

摘　　要：In eukaryotes,the nuclear membrane that encapsulates genomic DNA is composed of an inner nuclear membrane(INM),an outer nuclear membrane(ONM),and a perinuclear space.SUN proteins located in the INM and KASH proteins in the ONM form the SUN-KASH NM-bridge,which functions as the junction of the nucleocytoplasmic complex junction.Proteins containing the SUN domain showed the highest correlation with differentially accumulated proteins(DAPs)in the wheat response to fungal stress.To understand the characteristics of SUN and its associated proteins in wheat responding to pathogen stress,here we investigated and comprehensive analyzed SUN-and KASHrelated proteins among the DAPs under fungi infection based on their conserved motifs.In total,four SUN proteins,one WPP domain-interacting protein(WIP),four WPP domain-interacting tail-anchored proteins(WIT),two WPP proteins and one Ran GTPase activating protein(RanGAP)were identified.Following transient expression of Nicotiana benthamiana,TaSUN2,TaRanGAP2,TaWIT1 and TaWIP1 were identified as nuclear membrane proteins,while TaWPP1 and TaWPP2 were expressed in both the nucleus and cell membrane.RT-qPCR analysis demonstrated that the transcription of TaSUN2,TaRanGAP2 and TaWPP1 were strongly upregulated in response to fungal infection.Furthermore,using the bimolecular fluorescence complementation,the luciferase complementation and a nuclear and splitubiquitin-based membrane yeast two-hybrid systems,we substantiated the interaction between TaSUN2 and TaWIP1,as well as TaWIP1/WIT1 and TaWPP1/WPP2.Silencing of TaSUN2,TaRanGAP2 and TaWPP1 in wheat leaves promoted powdery mildew infection and hyphal growth,and reduced the expression of TaBRI1,TaBAK1 and Ta14-3-3,indicating that these NM proteins play a positive role in resistance to fungal stress.Our study reveals the characteristics of NM proteins and propose the preliminary construction of SUN-WIP-WPP-RanGAP complex in wheat,which represents a foundation for detail elucidating their functions in wheat in future.

关 键 词：Triticum aestivum SUN KASH Fungal stress 

分 类 号：S51[农业科学—作物学]