Signal Peptide Peptidase and PI4Kβ1/2 play opposite roles in plant ER stress response and immunity  

作　　者：Karen Thulasi Devendrakumar Tony ShengZhe Peng Leon Pierdzig Edan Jackson Volker Lipka Xin Li 

机构地区：[1]Michael Smith Laboratories,University of British Columbia,Vancouver,BC V6T 1Z4,Canada [2]Department of Botany,University of British Columbia,Vancouver,BC V6T 1Z4,Canada [3]Department of Plant Cell Biology,Georg August Universitat Gottingen,37077 Gottingen,Lower Saxony,Germany

出　　处：《Stress Biology》2024年第1期508-524,共17页逆境生物学(英文)

基　　金：supported by grants to XL from the National Sciences and Engineering Research Council(NSERC)Discovery program;the NSERCCREATE funded Plant Responses To Eliminate Critical Threats(PRoTECT)program,Canada Research Chairs(CRC);the Canadian Foundation for Innovation(CFI),and scholarships to KTD from the Izaak Walton Killam Memorial Fund of the Killam Trusts and The University of British Columbia’s Four Year Fellowship program。

摘　　要：The Arabidopsis pi4kβ1,2 mutant is mutated in the phosphatidylinositol 4-kinase(PI4K)β1 and PI4Kβ2 enzymes which are involved in the biosynthesis of phosphatidylinositol 4-phosphate(PI4P),a minor membrane lipid with important signaling roles.pi4kβ1,2 plants display autoimmunity and shorter roots.Though the pi4kβ1,2 mutant has been extensively characterized,the source of its autoimmunity remains largely unknown.In this study,through a genetic suppressor screen,we identified multiple partial loss-of-function alleles of signal peptide peptidase(spp)that can suppress all the defects of pi4kβ1,2.SPP is an intramembrane cleaving aspartic protease.Interestingly,pi4kβ1,2 plants display enhanced ER stress response and mutations in SPP can suppress such phenotype.Furthermore,reduced ER stress responses were observed in the spp single mutants.Overall,our study reveals a previously unknown function of PI4Kβand SPP in ER stress and plant immunity.

关 键 词：Signal peptide peptidase Plant immunity pi4kβ1 2 PI4Kβ ER stress EMS mutagenesis 

分 类 号：Q94[生物学—植物学]