Maize multi-omics reveal leaf water status controlling of differential transcriptomes,proteomes and hormones as mechanisms of age-dependent osmotic stress response in leaves  

作　　者：Liangjie Niu Wenkang Wang Yingxue Li Xiaolin Wu Wei Wang 

机构地区：[1]National Key Laboratory of Wheat and Maize Crop Science,College of Life Sciences,Henan Agricultural University,Zhengzhou 450046,China

出　　处：《Stress Biology》2024年第1期525-542,共18页逆境生物学(英文)

基　　金：supported by the National Natural Science Foundation of China(32272026 and U1904107 to W.W.);the National Key Laboratory of Wheat and Maize Crop Science,Henan agricultural University,China(39990101 to W.W.).

摘　　要：Drought-induced osmotic stress severely affects the growth and yield of maize.However,the mechanisms underlying the different responses of young and old maize leaves to osmotic stress remain unclear.To gain a systematic understanding of age-related stress responses,we compared osmotic-stress-induced changes in maize leaves of different ages using multi-omics approaches.After short-term osmotic stress,old leaves suffered more severe water deficits than young leaves.The adjustments of transcriptomes,proteomes,and hormones in response to osmotic stress were more dynamic in old leaves.Metabolic activities,stress signaling pathways,and hormones(especially abscisic acid)responded to osmotic stress in an age-dependent manner.We identified multiple functional clusters of genes and proteins with potential roles in stress adaptation.Old leaves significantly accumulated stress proteins such as dehydrin,aquaporin,and chaperones to cope with osmotic stress,accompanied by senescence-like cellular events,whereas young leaves exhibited an effective water conservation strategy mainly by hydrolyzing transitory starch and increasing proline production.The stress responses of individual leaves are primarily determined by their intracellular water status,resulting in differential transcriptomes,proteomes,and hormones.This study extends our understanding of the mechanisms underlying plant responses to osmotic stress.

关 键 词：Age-related changes Old and young leaves Osmotic stress PHYTOHORMONE Proteome Transcriptome Zea mays 

分 类 号：S51[农业科学—作物学]