DkWRKY transcription factors enhance persimmon resistance to Colletotrichum horii by promoting lignin accumulation through DkCAD1 promotor interaction  

作　　者：Hanyue Fan Xiaoxia Shen Yu Ding Yongkuan Li Shuyuan Liu Yong Yang Yuduan Ding Changfei Guan 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas,College of Horticulture,Northwest A&F University,Yangling,Shaanxi,China

出　　处：《Stress Biology》2024年第1期547-563,共17页逆境生物学(英文)

基　　金：supported by the National Natural Science Foundation of China(32272672 and 32302478);National Key Research and Development Program of China(2022YFD2200400 and 2019YFD1000600).

摘　　要：Persimmon anthracnose,a severe disease caused by the hemibiotrophic fungus Colletotrichum horii,poses a substantial threat to China’s persimmon industry.Previous research showed that‘Kangbing Jianshi’cultivar exhibits strong resistance to anthracnose.Notably,‘Kangbing Jianshi’branches exhibit greater lignification compared with the susceptible‘Fuping Jianshi’cultivar.In this study,higher lignin content was observed in‘Kangbing Jianshi’compared with‘Fuping Jianshi’,and this difference was associated with disease resistance.Transcriptome and metabolome analyses revealed that the majority of differentially expressed genes and differentially accumulated metabolites were primarily enriched in the phenylpropanoid biosynthesis and lignin synthesis pathways.Furthermore,significant upregulation of DkCAD1,a pivotal gene involved in lignin metabolism,was observed in the resistant cultivar when inoculated with C.horii.Transient overexpression of DkCAD1 substantially increased lignin content and improved resistance to C.horii in a susceptible cultivar.Furthermore,through yeast one-hybrid(Y1H)assays,we identified two WRKY transcription factors,DkWRKY8 and DkWRKY10,which interacts with the DkCAD1 promoter and induces its activity.Overexpression of DkWRKY8 and DkWRKY10 not only increased leaf lignin content but also enhanced persimmon tolerance to C.horii.Moreover,the expression levels of DkCAD1,DkWRKY8,and DkWRKY10 were significantly increased in response to salicylic acid and jasmonic acid in the resistant cultivar.These findings enhance our understanding of the molecular functions of DkWRKY8,DkWRKY10,and DkCAD1 in persimmons,as well as their involvement in molecular breeding processes in persimmons.

关 键 词：PERSIMMON DkCAD1 DkWRKY LIGNIN Colletotrichum horii SA JA 

分 类 号：S66[农业科学—果树学]