凡纳滨对虾不同组织对EHP的感染差异研究  

The Infection Differences of EHP in Different Tissues of Litopenaeus vannamei

作　　者：常林瑞 张兆新 何传瑜 曹政 司凌郡 闫冬春[1] CHANG Linrui;ZHANG Zhaoxin;HE Chuanyu;CAO Zheng;SI Lingjun;YAN Dongchun(School of Fisheries,Ludong University,Yantai 264039,China)

机构地区：[1]鲁东大学水产学院,山东烟台264039

出　　处：《鲁东大学学报(自然科学版)》2025年第1期25-30,共6页Journal of Ludong University:Natural Science Edition

基　　金：山东省自然科学基金面上项目(ZR2024MC115)。

摘　　要：近年来,虾肝肠胞虫在我国对虾养殖区具有较高的流行风险,已成为影响对虾养殖业健康发展的主要病原。为了解EHP的早期感染机制,本研究利用常规PCR和qPCR技术,对投喂攻毒10天的凡纳滨对虾5种组织进行EHP检测。结果显示:不同组织中EHP阳性率由高到低为(中肠与游泳足相同)、鳃、肝胰腺、肌肉,分别为70%、70%、60%、30%、10%;不同组织中EHP载量高低顺序为中肠、游泳足、鳃、肝胰腺、肌肉。这一结果表明,凡纳滨对虾在感染EHP早期阶段,多个组织可被感染,中肠是对EHP最为敏感的组织。In recent years,Enterocytozoon hepatopenaei(EHP)has a high epidemic risk in shrimp farming areas in China,and has become the main pathogen affecting the healthy development of shrimp aquaculture.To understand the early infection mechanism of EHP,this study employed conventional PCR and qPCR techniques to assess EHP in five tissues from Litopenaeus vannamei,which were orally challenged for 10 days.The results showed that the positive rates of EHP in different tissues from high to low were the same in midgut and swimming leg,gill,hepatopancreas and muscle,with rates of 70%,70%,60%,30%,and 10%,respectively;The EHP load was highest in the midgut,followed by the swimming leg,gill,hepatopancreas,and then muscle.This result indicates that in the early stages of EHP infection in juvenile shrimp,multiple tissues can be infected,with the midgut being the most sensitive tissue.

关 键 词：凡纳滨对虾 虾肝肠胞虫 不同组织 早期感染 

分 类 号：S945.4[农业科学—水产养殖]