Biogas upgrading towards acetic acid production using Clostridium thailandense supplemented with granular activated carbon(GAC)and L-arginine:A genomic analysis approach  

作　　者：Srisuda Chaikitkaew Nantharat Wongfaed Chonticha Mamimin Sompong O-Thong Alissara Reungsang 

机构地区：[1]Department of Biotechnology,Faculty of Technology,Khon Kaen University,Khon Kaen 40002,Thailand [2]Research Group for Development of Microbial Hydrogen Production Process from Biomass,Khon Kaen University,Khon Kaen 40002,Thailand [3]Biofuel and Biocatalysis Innovation Research Unit,Nakhonsawan Campus,Mahidol University,Nakhonsawan 60130,Thailand [4]Academy of Science,Royal Society of Thailand,Bangkok 10300,Thailand

出　　处：《Carbon Resources Conversion》2024年第4期1-9,共9页碳资源转化(英文)

基　　金：funding from the Post-Doctoral Training Program from Khon Kaen University,Thailand(Grant No.PD2566-01).

摘　　要：This study explores the impact of granular activated carbon (GAC) and L-arginine supplementation on biogas upgrading and acetic acid production employing Clostridium thailandense. GAC and L-arginine concentrations ranged from 0 to 20 g/L and 0 to 5 g/L, respectively, with H_(2) acting as the electron donor at an H_(2) to CO_(2) ratio of 2:1 (v/v). Experiments were conducted at 30 ℃ with an agitation speed of 150 rpm. Additionally, gene annotation of the C. thailandense genome using Rapid Annotations using Subsystems Technology (RAST) identified genes involved in CO_(2) to acetic acid conversion. Results indicate that adding 7.5 g/L GAC boosts CH4 purity in biogas, elevating CO_(2) and H_(2) consumption efficiencies to 88.3 % and 98.7 %, respectively. This enhancement leads to a CH4 content increase to 93.3 %, accompanied by 0.90 g/L acetic acid production. Conversely, L-arginine demonstrates no significant impact on CO_(2) conversion. Leveraging RAST, the study identifies hydrogenase genes and NADH-dependent ferredoxin-NADP^(+) oxidoreductase (Nfn), as crucial for heightened H_(2) consumption efficiencies and cell growth facilitated by GAC, thus enhancing biogas upgrading efficiency in C. thailandense. This research provides vital insights into optimizing sustainable biogas production through strategic GAC utilization and elucidates the roles of hydrogenase genes and Nfn.

关 键 词：Biogas transformation Carbon capture and utilization CO_(2) consumption efficiency CO_(2) utilization Sustainable biogas applications 

分 类 号：F42[经济管理—产业经济]