A multienzyme system immobilized on surface-modified metal–organic framework for enhanced CO_(2) hydrogenation  

作　　者：Shadeera Rouf Yaser E.Greish Bart Van der Bruggen Sulaiman Al-Zuhair 

机构地区：[1]Department of Chemical and Petroleum Engineering,UAE University,15551 Al Ain,UAE [2]Department of Chemistry,UAE University,15551 Al Ain,UAE [3]Department of Chemical Engineering,KU Leuven,3000 Leuven,Belgium

出　　处：《Carbon Resources Conversion》2024年第4期31-42,共12页碳资源转化(英文)

基　　金：support from the College of Graduate Studies (fund number 31N438);UAEU and Zayed Center of Health Sciences (grant number 12R077) to carry out this study.

摘　　要：Hydrogenating carbon dioxide to formate using formate dehydrogenase (FDH) is a sustainable approach for CO_(2) mitigation. Herein, we developed a biocatalytic system with cofactor regeneration by immobilizing multiple enzymes, namely FDH, carbonic anhydrase (CA), and glutamate dehydrogenase (GDH), on a hydrophobic surface modified MOF, SA-HKUST-1. The adsorption kinetics of the multiple enzymes on the SA-HKUST-1 surface were described using pseudo second-order model, while the equilibrium followed Freundlich isotherm. Formate production by the enzymes immobilized on SA-HKUST-1 was 3.75 times higher than that achieved by free enzymes and 8.4 times higher than that of FDH immobilized alone on SA-HKUST-1. The hydrophobic interaction between the enzymes and the support altered the secondary structure of enzymes, and the immobilized enzymes retained 94% of their activity after four reuse cycles. This study provides novel insights into the combined effect of hydrophobic support and multiple enzymes on the catalytic efficiency and stability of FDH. These findings can provide a basis for developing a highly stable biocatalytic system with cofactor regeneration for continuous hydrogenation of CO_(2) to formate at the industrial level.

关 键 词：CO_(2) hydrogenation Multienzyme system Hydrophobic metal-organic framework HKUST-1 Cofactor regeneration FORMATE 

分 类 号：O64[理学—物理化学]