基于微透析探究付罐疗法对丹参“水-脂”二元组分的促渗作用  

Exploring the Promoting Effect of Fu's Cupping Therapy on the"Water-Lipid"Binary Component of Salviae Miltiorrhizae Radix et Rhizoma Based on Microdialysis

作　　者：谢欢 周斌 何金英 徐剑 刘耀 曹国琼 张永萍 XIE Huan;ZHOU Bin;HE Jinying;XU Jian;LIU Yao;CAO Guoqiong;ZHANG Yongping(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Guizhou Institute of Engineering and Technology for Preparation and Preparation of Traditional Chinese Medicine,Guiyang 550025,China;National Engineering and Technology Research Center of Miao Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵阳550025 [2]贵州省中药民族药炮制与制剂工程技术研究中心,贵阳550025 [3]国家苗药工程技术研究中心,贵阳550025

出　　处：《中国现代应用药学》2024年第24期3537-3542,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(81873020);贵州省高等学校中药民族药(苗药)新剂型新制剂工程研究中心项目(黔教技[2022]022);国家苗药工程技术研究中心能力提升项目(黔科合中引地[2023]006);贵州省高层次创新型人才项目(黔科合平台人才-GCC[2023]037)。

摘　　要：目的建立丹参“水-脂”二元组分的经皮微透析方法,研究付罐疗法(Fu's cupping therapy,FCT)不同处理方式的在体经皮促渗特征。方法考察灌流流速、温度和药物浓度对探针回收率的影响,采用增量法与减量法探究探针膜内外渗透性的一致性,考察探针体内外稳定性;以新西兰兔为动物模型,测定FCT不同处理时间(背部留罐5、10、15 min)对丹参水溶性成分与脂溶性成分的皮肤动力学行为。结果流速越大,回收率越低;温度越高,回收率越高;在不同药物浓度下,探针回收率与损失率近似相等;且探针回收率在10 h内稳定性良好。FCT背部留罐15 min对水溶性成分具有显著促渗作用,FCT背部留罐15 min和10 min对丹参脂溶性成分经皮促渗效果明显。结论FCT可作为一种新型的物理促渗技术。OBJECTIVE To establish the method of percutaneous microdialysis of"Water-lipid"binary components of Salviae Miltiorrhizae Radix et Rhizoma,and to study the characteristics of percutaneous penetration enhancement in vivo in different processing method of Fu's cupping therapy(FCT).METHODS The influence of perfusion velocity,temperature and drug concentration on the recovery rate of the probe was investigated.The consistency of the permeability of the probe in vitro and in vivo was investigated by incremental and decrement methods.The skin kinetics of water-soluble and fat-soluble components of Salviae Miltiorrhizae Radix et Rhizoma was studied under different FCT treatment times(FCT on the back for 5,10 and 15 min).RESULTS The higher the flow rate,the lower the recovery rate;the higher the temperature,the higher the recovery rate.Under different drug concentrations,the recovery rate and the loss rate of the probe were approximately equal;and the recovery rate of the probe was stable within 10 h.FCT on the back for 15 min had a significant effect on the permeation of water-soluble components,and FCT on the back for 15,10 min had a significant effect on the transdermal permeation of the fat-soluble components of Salviae Miltiorrhizae Radix et Rhizoma.CONCLUSION The FCT technique can be used as a new physical penetration technique.

关 键 词：付罐 物理促渗技术 微透析技术 水溶性成分 脂溶性成分 丹参 

分 类 号：R943[医药卫生—药剂学]