High-throughput transport-of-intensity quantitative phase imaging with aberration correction  

作　　者：Linpeng Lu Shun Zhou Yefeng Shu Yanbo Jin Jiasong Sun Ran Ye Maciej Trusiak Peng Gao Chao Zuo 

机构地区：[1]Smart Computational Imaging Laboratory(SCILab),School of Electronic and Optical Engineering,Nanjing University of Science and Technology,Nanjing,Jiangsu Province 210094,China [2]Smart Computational Imaging Research Institute(SCIRI)of Nanjing University of Science and Technology,Nanjing,Jiangsu Province 210019,China [3]Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense,Nanjing,Jiangsu Province 210094,China [4]School of Computer and Electronic Information,Nanjing Normal University,Nanjing 210023,China [5]Institute of Micromechanics and Photonics,Warsaw University of Technology,8 Sw.A.Boboli St.,Warsaw 02-525,Poland [6]School of Physics,Xidian University,Xi'an,China

出　　处：《Light(Advanced Manufacturing)》2024年第4期54-63,共10页光(先进制造)(英文)

基　　金：supported by the National Natural Science Foundation of China(62227818,62105151,62175109,U21B2033,62105156,62361136588);National Key Research and Development Program of China(2022YFA1205002);Leading Technology of Jiangsu Basic Research Plan(BK20192003);Youth Foundation of Jiangsu Province(BK20210338);Biomedical Competition Foundation of Jia-ngsu Province(BE2022847);Key National Industrial Technology Cooperation Foundation of Jiangsu Province(BZ2022039);Fundamental Research Funds for the Central Universities(30920032101,30923010206);Fundamental Scientific Research Business Fee Funds for the Central Universities(2023102001);Open Research Fund of Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense(JSGP202105,JSGP202201);National Science Center,Poland(2020/37/B/ST7/03629).

摘　　要：The transport of intensity equation(TIE)is a well-established phase retrieval technique that enables incoherent diffraction limit-resolution imaging and is compatible with widely available brightfield microscopy hardware.However,existing TIE methods encounter difficulties in decoupling the independent contributions of phase and aberrations to the measurements in the case of unknown pupil function.Additionally,spatially nonuniform and temporally varied aberrations dramatically degrade the imaging performance for long-term research.Hence,it remains a critical challenge to realize the high-throughput quantitative phase imaging(QPI)with aberration correction under partially coherent illumination.To address these issues,we propose a novel method for highthroughput microscopy with annular illumination,termed as transport-of-intensity QPI with aberration correction(TI-AC).By combining aberration correction and pixel super-resolution technique,TI-AC is made compatible with large pixel-size sensors to enable a broader field of view.Furthermore,it surpasses the theoretical Nyquist-Shannon sampling resolution limit,resulting in an improvement of more than two times.Experimental results demonstrate that the half-width imaging resolution can be improved to~345 nm across a 10×field of view of 1.77 mm^(2)(0.4 NA).Given its high-throughput capability for QPI,TI-AC is expected to be adopted in biomedical fields,such as drug discovery and cancer diagnostics.

关 键 词：Transport of intensity Quantitative phase microscopy Aberration correction High-throughput imaging 

分 类 号：O43[机械工程—光学工程]