山东省生猪养殖与屠宰环节猪圆环病毒流行情况调查  

Epidemiological Investigation on Porcine Circovirus in the Processes of Pig Production and Slaughter in Shandong Province

作　　者：李法凯 刘存 韩小龙 兰邹然[1] 陈伟 邵新宇 郭晓敏 王贵升[1] 刘砚涵 孙圣福[1] Li Fakai;Liu Cun;Han Xiaolong;Lan Zouran;Chen Wei;Shao Xinyu;Guo Xiaomin;Wang Guisheng;Liu Yanhan;Sun Shengfu(Shandong Center for Animal Disease Prevention and Control,Shandong Center for Epidemiological Investigation and Surveillance of Zoonotic Diseases,Jinan 250100,Shandong,China;Shengli Township Animal Husbandry and Veterinary Station,Tancheng 276100,Shandong,China;Jinan Center for Animal Disease Prevention and Control,Jinan 250000,Shandong,China)

机构地区：[1]山东省动物疫病预防与控制中心,山东省人畜共患病流调监测中心,山东济南250100 [2]郯城县胜利畜牧兽医站,山东郯城276100 [3]济南市动物疫病预防与控制中心,山东济南250000

出　　处：《中国动物检疫》2025年第1期23-28,共6页China Animal Health Inspection

基　　金：山东省重点研发计划“猪重要疫病基因工程疫苗创制”项目(2023CXGC010705)。

摘　　要：为了解山东省猪圆环病毒(porcine circoviruses,PCV)流行情况,对采自山东省16个地市的191份样品进行猪圆环病毒2型(PCV2)和猪圆环病毒3型(PCV3)检测,并对阳性样品进行ORF2基因测序及遗传演化分析。结果显示;PCV2的个体阳性率和场群阳性率分别为19.90%(38/191)和68.75%(22/32),PCV3分别为13.09%(25/191)和62.50%(20/32),PCV3和PCV2混合个体阳性率为5.24%(10/191);有11个地市检出PCV2,10个地市检出PCV3;从不同环节来看,养殖环节的PCV2、PCV3个体阳性率分别为16.25%、8.75%,屠宰环节分别为38.71%、35.48%,屠宰环节的PCV2、PCV3个体阳性率及混合阳性率均显著高于养殖场(P<0.05);遗传演化分析显示,PCV2属PCV2d基因亚型,PCV3属PCV3c基因亚型。结果说明;山东省猪群中存在PCV2和PCV3的共同流行且分布广泛,屠宰环节的感染情况较养殖环节严重;PCV2流行毒株与我国当前流行毒株优势基因型相符。建议持续开展PCV监测工作,追踪PCV变异情况,重点加强屠宰场的消毒灭源工作,防控PCV传播。In order to investigate the prevalence of porcine circovirus(PCV)in Shandong Province,191 samples collected from 16 prefecture-level cities in the province were detected for PCV type 2(PCV2)and type 3(PCV3),and ORF2 gene sequencing and genetic evolution analysis were performed for the positive samples.The results showed that the positive rates at the individual and farm/population level were 19.90%(38/191)and 68.75%(22/32)for PCV2,respectively,and 13.09%(25/191)and 62.50%(20/32)for PCV3,respectively,and the mixed individual positive rate of PCV3 and PCV2 was 5.24%(10/191);PCV2 was detected in 11 cities and PCV3 was in 10 cities;the individual positive rates of PCV2 and PCV3 were 16.25%and 8.75%in production process,respectively,and 38.71%and 35.48%in slaughter process,respectively,the individual and mixed positive rates of PCV2 and PCV3 were obviously higher in slaughter process than those in farms(P<0.05);genetic evolution analysis showed that PCV2 and PCV3 belonged to PCV2d and PCV3c gene subtypes,respectively.In conclusion,PCV2 and PCV3 were co-prevalent and widely distributed in pigs in Shandong Province,in particular,more seriously in slaughter process than in production process;and the epidemic strain of PCV2 was consistent with the dominant genotype in China.Therefore,authorities should continuously carry out PCV monitoring and trace for its variation,giving a priority to strengthen disinfection and elimination of disease sources in slaughterhouses to prevent and control any transmission.

关 键 词：猪圆环病毒2型 猪圆环病毒3型 流行病学调查 山东省 

分 类 号：S855.3[农业科学—临床兽医学]