基于免疫学方法分离哺乳动物X、Y精子研究进展  

Advances in the Study of Isolation of Mammal X and Y Sperm Based on Immunological Methods

作　　者：杨海涛 牛隽 卫利选 朱大伟 胡张涛 曹平 贾志宏 胡建宏[3] YANG Haitao;NIU Juan;WEI Lixuan;ZHU Dawei;HU Zhangtao;CAO Ping;JIA Zhihong;HU Jianhong(Animal Husbandry Industry Experiment and Demonstration Center of Shaanxi Province,Shaanxi Jingyang 713702,China;Xi'an animal husbandry technology extension center breeding bull station,Shaanxi Xi’an 710000,China;College of Animal Science and Technology,Northwest A&F University,Shaanxi Yangling 712100,China)

机构地区：[1]陕西省畜牧产业试验示范中心,陕西泾阳713702 [2]西安市畜牧技术推广中心种公牛站,陕西西安710000 [3]西北农林科技大学,陕西杨凌712100

出　　处：《中国畜牧杂志》2025年第1期81-85,共5页Chinese Journal of Animal Science

基　　金：西安市科技计划项目(21NYYF0014);陕西省农业领域一般项目(2023YBNY139)。

摘　　要：高效准确的性别控制技术对畜牧业生产具有重要的实用价值和现实意义。利用X、Y精子之间的差异将X精子与Y精子分离,使用分离后的精子进行人工授精从而改变动物后代性别比例,是目前最为有效的性别控制方法之一。免疫学分离法即利用X、Y精子间的差异表达蛋白,根据抗原抗体结合或激活特定蛋白来改变某一类精子的运动能力进而分离精子的方法,具有高效、廉价、对精子损伤低等优点,是性别控制技术领域的研究热点。本文对哺乳动物X、Y精子差异表达蛋白以及利用免疫学方法分离X、Y精子的研究进展进行综述,旨在为X、Y精子分离技术以及性控精液分离方法的建立提供参考。Efficient and accurate gender control technology has important practical value and relevance to livestock production.One of the most effective methods of sex control is to separate X sperm from Y sperm by utilizing the differences between X and Y sperm,and use the separated sperm for artificial insemination to change the sex ratio of animal offspring.Immunological separation method is a method that use the differentially expressed proteins between X and Y spermatozoa to change the motility of a certain type of spermatozoa based on antigen-antibody binding or activation of specific proteins to separate the spermatozoa,has the advantages of being highly efficient,inexpensive,and causing low damage to spermatozoa,and it is a hot spot of research in the field of gender control technology.This paper reviews the progress of research on mammalian X and Y spermatozoa differentially expressed proteins as well as the separation of X and Y spermatozoa using immunological methods,with the aim of providing a reference for X and Y spermatozoa isolation techniques as well as the establishment of sexually controlled semen isolation methods.

关 键 词：精子分离 性控精液 免疫学方法 差异表达蛋白 

分 类 号：S814[农业科学—畜牧学]