山东鸭源粪肠球菌的分离鉴定及药物敏感性分析  

Isolation,Identification and Drug Sensitivity Analysis of Enterococcus faecalis from Duck Source in Shandong Province

作　　者：刘洪港 张筱 赵杰 石艳红 王振 李玉峰[2] 汪建华 LIU Honggang;ZHANG Xiao;ZHAO Jie;SHI Yanhong;WANG Zhen;LI Yufeng;WANG Jianhua(Shandong Hekangyuan Biological Breeding Co.,Ltd.,Jinan 250100,China;Poultry Research Institute of Shandong Academy of Agricultural Sciences/Poultry Breeding and Disease Prevention and Control Shandong Engineering Research Center,Jinan 250100,China)

机构地区：[1]山东和康源生物育种股份有限公司,山东济南250100 [2]山东省农业科学院家禽研究所/家禽育种与疫病防控山东省工程研究中心,山东济南250100

出　　处：《家禽科学》2025年第2期26-31,I0006,共7页China Poultry Science

摘　　要：为探究引起山东省某规模肉鸭养殖场雏鸭死亡的原因,本试验在病死鸭病变肝脏组织中分离到疑似粪肠球菌病原菌。通过细菌分离培养、形态观察、生化试验鉴定、16S rRNA序列分析鉴定病原菌,对分离菌进行抗菌药物敏感性试验,以便精准用药防控。结果显示:分离菌为粪肠球菌,且该菌株多重耐药性较强,对阿莫西林、安普霉素敏感,对头孢噻肟、氟苯尼考、卡那霉素、恩诺沙星、氨苄西林、多黏菌素耐药。本研究为鸭源粪肠球菌的临床防控提供了参考依据。To investigate the etiology of duck mortality in a duck farm located in Shandong Province,pathogenic bacteria suspected to be Enterococcus were isolated from the liver tissues of deceased ducks.For precise pharmacological intervention,a comprehensive approach was employed,including bacterial isolation,morphological observation,biochemical testing,and 16S rRNA sequence analysis for pathogen identification.Additionally,drug sensitivity assays were performed on the isolated pathogens.The results indicated that the isolated strain was identified as Enterococcus faecalis,exhibiting significant multi-drug resistance.The strain demonstrated sensitivity to Amoxicillin and Ampramycin,while showing resistance to Cefotaxime,Flufenicol,Kanamycin,Enrofloxacin,Ampicillin,and Polycolistin.This study offers valuable insights for the clinical prevention and control of Enterococcus faecalis originating from ducks.

关 键 词：粪肠球菌 分离鉴定 序列分析 最小抑菌浓度 

分 类 号：S858.32[农业科学—临床兽医学]