Recent advances in CRISPR/Cas systems-associated surface enhanced Raman spectroscopy biosensor towards nucleic acid detection  

作　　者：Dongjie Zhang Siyuan He Zixu Wang Peirao Yan Huandi Li Xinyi Xu Qi Zeng Nan Wang Xueli Chen 

机构地区：[1]Biomedical Photonics and Molecular Imaging Laboratory,School of Life Science and Technology,Xidian University and Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information,Xi'an,Shaanxi,710126,China [2]Engineering Research Center of Molecular and Neuro Imaging,Ministry of Education&Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province,Xidian University,Xi'an,Shaanxi,710126,China [3]Innovation Center for Advanced Medical Imaging and Intelligent Medicine,Guangzhou Institute of Technology,Xidian University,Guangzhou,Guangdong,51055,China

出　　处：《Medicine in Novel Technology and Devices》2024年第4期137-145,共9页医学中新技术与新装备(英文)

基　　金：This work was supported by the National Key Research and Development Program of China,grants No.2022YFB3203800;the National Natural Science Foundation of China,grants 32201133 and 62275210;the National Young Talent Program;the Young Talent Fund of Association for Science and Technology in Shaanxi,grants 20230222;the Key Research and Development Program of Shaanxi,grant 2023-YBSF-143 and 2021ZDLSF04-05;the Science and Technology Projects in Guangzhou,grants 2023A04J1509;the Fundamental Research Funds for Central Universities,grants KYFZ24006.

摘　　要：Nucleic acids play a decisive role in vital life processes,such as growth,heredity,and evolution,and exploring different types of nucleic acid detection techniques has always been a research hotspot worldwide.Compared to traditional nucleic acid analysis methods,surface-enhanced Raman spectroscopy(SERS)detection has advantages,such as high sensitivity,good specificity,narrow spectral bands,minimal sample requirement,simple operation,and resistance to interference from autofluorescence and water in biological samples.The clustered regularly interspaced short palindromic repeats(CRISPR)/Cas system has gained widespread attention in the fields of gene editing and biosensors.Combining the sequence-specific recognition capability of the CRISPR/Cas system with the high sensitivity fingerprinting properties of SERS technology offers a simple,rapid,and sensitive method towards nucleic acid detection.Here,common SERS Nanotags methods for nucleic acid detection are introduced,including“sandwich assay”,“signal switch assay”and HCR signal amplification.Then,the building mechanism of CRISPR-associated SERS detection is summarized.CRISPR/Cas12a activated by crRNA can recognize and lock the complementary target double-stranded DNA(dsDNA),followed by ciscleavage of the dsDNA and trans-cleavage of the adjacent linker ssDNA.The loss of SERS signal based on aggregation is finally used for highly sensitive detection of the target DNA.Lastly,some typical applications through the integration of CRISPR/Cas and SERS technology are introduced,such as HIV-1 dsDNA,HBV DNA and AFB1 etc.

关 键 词：SPECTROSCOPY SANDWICH gained 

分 类 号：O65[理学—分析化学]