Earthworms and warming alter methane uptake and methane-cycling microbial community in meadow soil  

作　　者：Yujuan Kang Haitao Wu Qiang Guan Zhongsheng Zhang Wenfeng Wang 

机构地区：[1]State Key Laboratory of Black Soils Conservation and Utilization,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130102,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Key Laboratory of Wetland Ecology and Environment,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130102,China

出　　处：《Soil Ecology Letters》2024年第4期53-66,共14页土壤生态学快报(英文)

基　　金：funding from the National Natural Science Foundation of China under Grant No.U20A2083;from the Science and Technology Development Program of Jilin Province under Grant Nos.JL2022-12,20210509037RQ,and 20230101348JC.

摘　　要：Earthworms significantly reduced soil CH_(4) uptake at both temperatures,and warming significantly promoted soil CH_(4) uptake.Earthworms significantly altered methanotroph community,and warming significantly altered methanogen community,and their interaction had a significant influence on both methanogen and methanotroph communities.Soil properties exhibited a negative impact on CH_(4) uptake,while theα-diversity of methanotrophs was associated with enhanced CH_(4) uptake.Dissolved organic carbon(DOC)was identified as the most essential factor in forecasting soil CH_(4) uptake.The function and service of biologically driven ecosystems are undergoing significant changes due to climate warming.Earthworms play a crucial role as soil engineer by modulating the effects of climate change on soil nutrient cycle through alterations to biotic and abiotic soil conditions.However,there is currently a scarcity of information regarding the impacts of earthworms and warming on soil CH_(4) uptake and their associated microbial mechanisms.This study conducted a 61-day microcosm experiment to investigate the impact of warming(temperature rise from 14.2℃ to 17.2℃)and the presence of earthworms(Eisenia fetida and Moniligaster japonicus)on soil CH_(4) uptake.We employed gas chromatography and high-throughput sequencing to investigate the fluctuations in soil CH_(4) uptake and the microbial communities involved in methane cycling.Compared to low temperature conditions(14.2℃),we observed that warming significantly increased soil CH_(4) uptake in all treatments(nonearthworm:51.85%;Eisenia fetida:50.88%;Moniligaster japonicus:71.78%).Both Eisenia fetida and Moniligaster japonicus significantly reduced soil CH_(4) uptake at two temperatures compared to the non-earthworm treatment.Nevertheless,no significant impacts were found on soil CH_(4) uptake due to the interactions between earthworms and warming.The methanotroph communities exhibited notable variations among earthworm treatments,whereas the methanogenic communities displaye

关 键 词：endogeic earthworms epigeic earthworms greenhouse gas emissions Illumina sequencing network analysis 

分 类 号：S812.2[农业科学—草业科学]