Sporocarp-associated fungal co-occurrence networks in a corn field revealed by long-read high-throughput sequencing  

作　　者：Teng Yang Luyao Song Xu Liu Xia Luo Qiuyan Tan Cunzhi Zhang Jonathan MAdams Haiyan Chu 

机构地区：[1]State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Department of Microbiology,College of Life Sciences,Nanjing Agricultural University,Key Laboratory of Agricultural and Environmental Microbiology,Ministry of Agriculture and Rural Affairs,Nanjing 210095,China [4]School of Biology and Food Engineering,Chuzhou University,Chuzhou 239000,China [5]School of Geographic and Oceanographic Sciences,Nanjing University,Nanjing 210008,China

出　　处：《Soil Ecology Letters》2024年第4期99-111,共13页土壤生态学快报(英文)

基　　金：supported by the National Program on Key Basic Research Project(Grant No.2022YFD1500202);the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA28020202);the National Natural Science Foundation of China(Grant No.42277308).

摘　　要：We identified a sporocarp as Agrocybe dura growing next to a living corn using PacBio sequencing.The mycoparasitism of Trichoderma spp.on A.dura were revealed by the co-occurrence network analysis.For long-read HTS data,we updated a bioinformatic pipeline to enhance fungal taxonomic resolution.In forests,fungal sporocarps house the diverse fungicolous fungi;however,the relationships of sporocarps and associated fungal communities are rarely explored in agroecosystems.In a corn field near Gongzhuling City,Jilin Province,China,we found an epigeous sporocarp with agaricoid morphology that could grow next to the living corn plants.Using PacBio metabarcoding combined with an updated bioinformatic pipeline,we surveyed the fungal community profile along its cap,rhizomorph and hyphosphere soil at a much-improved taxonomic resolution.We identified the sporocarp,at a high probability,as Agrocybe dura,and this mushroom was significantly negatively correlated with Trichoderma hamatum and T.harzianum in the co-occurrence network.Fungal diversity in hyphosphere habitat was significantly higher than that in cap and rhizomorph habitats.Consistent with the pattern in fungal diversity,the node number,edge number,network diameter and average degree were significantly higher in hyphosphere habitat than other habitats.However,both the negative and positive cohesion were significantly higher in rhizomorph habitat than other habitats.Moreover,the z-c plot identified A.dura as the only network hub,linking multiple fungal species.The results give us a glimpse of the ecological relevance of saprobic mushrooms across the extensive northeastern black soil region of China.Our findings will aid in the assessment and forecasting of fungal diversity hotspots and their relationships with soil fertility in the‘Golden Corn Belt’of northeast China.

关 键 词：PacBio metabarcoding saprobic mushroom species identification co-occurrence network corn field northeast China 

分 类 号：S154.3[农业科学—土壤学]