The combined application of ethylenediurea and arbuscular mycorrhizal fungi alleviates ozone damage to Medicago sativa L.  

作　　者：Rongbin Yin Zhipeng Hao Xiangyang Yuan Xin Zhang Siyu Gun Xuemei Hu Lifan Wang Baodong Chen 

机构地区：[1]State Key Laboratory of Urban and Regional Ecology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]School of Applied Meteorology,Nanjing University of Information Science&Technology,Nanjing 210044,China [4]Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China [5]College of Landscape Architecture and Forestry,Qingdao Agricultural University,Qingdao 266109,China

出　　处：《Soil Ecology Letters》2024年第4期309-325,共17页土壤生态学快报(英文)

基　　金：funded by the National Key Research and Development Program of China(Grant No.2017YFE0127700);the Key Research and Development Program of Shandong Province,China(Grant No.2021CXGC010803).

摘　　要：EDU reduces O_(3) sensitivity of alfalfa by mediating antioxidant enzyme activities.AM symbiosis increases stomatal conductance and plant O_(3) sensitivity.AM fungi increase stomatal conductance by increasing plant stomatal density.AM inoculation combined with EDU can mitigate negative effects of O_(3) on plants.Ozone(O_(3))is a phytotoxic air pollutant,both ethylenediurea(EDU)and arbuscular mycorrhizal(AM)fungi can affect plant O_(3) sensitivity.However,the underlying mechanisms of EDU and AM fungi on plant O_(3) sensitivity are unclear,and whether the combined application of the two can alleviate O_(3) damage has not been verified.In this study,an open-top chamber experiment was conducted to examine the effects of EDU and AM inoculation on growth and physiological parameters of alfalfa(Medicago sativa L.)plants under O_(3) enrichment.The results showed that EDU significantly decreased O_(3) visible injury(28.67%−68.47%),while AM inoculation significantly increased O_(3) visible injury.Mechanistically,the reduction of plant O_(3) sensitivity by EDU was mediated by antioxidant enzyme activities rather than stomatal conductance.Although AM inoculation increased antioxidant enzyme activities(4.99%−211.23%),it significantly increased stomatal conductance(42.69%)and decreased specific leaf weight(12.98%),the negative impact was overwhelming.Therefore,AM inoculation increased alfalfa’s O_(3) sensitivity.Furthermore,we found AM inoculation increased stomatal conductance by increasing stomatal density.The research indicated EDU was sufficient to counteract the negative effects of AM inoculation on O_(3) sensitivity.The combined application of EDU and AM fungi could largely alleviate the adverse effects of O_(3) on plant performance.

关 键 词：ozone sensitivity antioxidant enzyme stomatal conductance specific leaf weight ethylenediurea(EDU) ALFALFA 

分 类 号：S344.2[农业科学—作物栽培与耕作技术]