ChREBP网络成员及TUG1在宫腔粘连中的表达及意义  

Expression and significance of ChREBP network members and TUG1 in intrauterine adhesion

作　　者：朱玲静 陈明庆 严子璇 闫雪兰[4] 艾瑛[4] ZHU Lingjing;CHEN Mingqing;YAN Zixuan;YAN Xuelan;AI Ying(Medical School,Kunming University of Science and Technology/Yunnan Provincial First People’s Hospital,Kunming,Yunnan 650500,China;Hospital of Guizhou Panjiang Coal and Electricity Group Co.,Ltd.,Liupanshui,Guizhou 553000,China;Department of Gynecology,Yueyang Municipal People’s Hospital,Yueyang,Hunan 414000,China;Department of Obstetrics and Gynecology,Yunnan Provincial First People’s Hospital/Affiliated Hospital of Kunming University of Science and Technology/National Key Clinical Specialty of Gynecology/Yunnan Province Clinical Research Center for Gynecological and Obstetric Diseases,Kunming,Yunnan 650000,China)

机构地区：[1]昆明理工大学医学院/云南省第一人民医院,昆明650500 [2]贵州盘江煤电集团有限责任公司医院,贵州六盘水553000 [3]岳阳市人民医院妇科,湖南岳阳414000 [4]云南省第一人民医院妇产科/昆明理工大学附属医院/妇科国家级临床重点专科/云南省妇产疾病临床医学研究中心,昆明650000

出　　处：《重庆医学》2025年第1期7-11,17,共6页Chongqing Medical Journal

基　　金：云南省科学技术厅-昆明医科大学应用基础研究联合专项资金(202101AY070001-261);妇科国家级临床重点专科开放课题(2022FKZDZK-14);云南省妇产疾病临床医学研究中心开放课题(2022YJZX-FC12)。

摘　　要：目的探讨碳水化合物反应元件结合蛋白(ChREBP)网络成员[ChREBP、Max样蛋白X(Mlx)、癌基因相关因子(Max)、最大二聚蛋白1(Mxd1)、组蛋白去乙酰化酶1(HDAC1)]及牛磺酸上调基因1(TUG1)在宫腔粘连(IUA)中的表达及意义。方法选择10只8周龄雌性SD大鼠为研究对象。采用机械刮宫+脂多糖感染构建IUA大鼠模型作为IUA组(n=5),对照组宫腔不做任何操作(n=5);采用HE、Masson染色分别评估子宫内膜腺体数量及纤维化程度;采用Western blot法检测细胞核与细胞质中ChREBP网络成员蛋白相对表达水平;采用实时荧光定量PCR(RT-qRCR)检测TUG1 mRNA相对表达水平。结果与对照组相比,IUA组子宫内膜腺体数量减少(P<0.05),纤维化面积百分比增加(P<0.05),细胞核中ChREBP网络成员的蛋白相对表达水平下调,细胞质中ChREBP网络成员的蛋白相对表达水平上调,TUG1的mRNA相对表达水平上调(P<0.05)。结论ChREBP核易位受阻并减少其下游网络成员的招募可能导致对TUG1转录的抑制作用减弱,从而促进IUA的发生。Objective To investigate the expression and significance of carbohydrate response element-binding protein(ChREBP)network members(ChREBP,Mlx,Max,Mxd1,HDAC1)and taurine-upregulated gene 1(TUG1)in intrauterine adhesion(IUA).Methods Ten female SD rats with 8 weeks old were selected as the study objects.The mechanical curettage plus lipopolysaccharide infection were used to construct the IUA rat model as the IUA group(n=5)and the uterine cavity of the control group did not perform any operation(n=5);HE and Masson staining were respectively used to assess the number of endometrial glands and the degree of fibrosis;Western blot was used to detect the expression level of ChREBP network members in the nucleus and cytoplasm;RT-qRCR was used to detect the expression level of TUG1.Results Compared with the control group,the number of endometrial glands in the IUA group was decreased(P<0.05),the percentage of the fibrotic area was increased(P<0.05),the protein expression level of ChREBP network members in the nucleus was down-regulated,the protein expression level of ChREBP network members in the cytoplasm was up-regulated and the mRNA relative expression level of TUG1 was up-regulated(P<0.05).Conclusion Blocked nuclear translocation of ChREBP and reduced the recruitment of its downstream network members may lead to attenuate the repressive effect on TUG1 transcription,thereby promoting IUA occurrence.

关 键 词：宫腔粘连 牛磺酸上调基因1 碳水化合物反应元件结合蛋白 机械刮宫 脂多糖感染 

分 类 号：R711.74[医药卫生—妇产科学]