子宫颈鳞状上皮内病变中PYCR2、CA125及ATG-12的表达及意义  

Expression and significance of PYCR2,CA125 and ATG-12 in squamous intraepithelial lesions of the uterine cervix

作　　者：朱兴华[1] 张邢松 沈蓉[1] 卫颖泽[1] ZHU Xinghua;ZHANG Xingsong;SHEN Rong;WEI Yingze(Department of Pathology,Nantong Tumor Hospital,Jiangsu Nantong 226361,China)

机构地区：[1]南通市肿瘤医院病理科,江苏南通226361

出　　处：《中国妇幼健康研究》2025年第1期69-73,共5页Chinese Journal of Woman and Child Health Research

基　　金：南通市卫健委科研项目(MB2020019)。

摘　　要：目的探讨吡咯啉-5-羧酸还原酶2(PYCR2)、糖类抗原125(CA125)、自噬相关蛋白-12(ATG-12)在子宫颈鳞状上皮内病变(SIL)诊断中的价值。方法于2020年1月至2021年12月在南通市肿瘤医院收集子宫颈组织样本共336例,其中正常子宫颈131例,低级别鳞状上皮内病变(LSIL)91例,高级别鳞状上皮内病变(HSIL)114例。分别检测各组中PYCR2、CA125、ATG-12的表达水平,并分析PYCR2、CA125、ATG-12与SIL的相关性及三者联合检测在子宫颈SIL诊断中的价值。结果LSIL组及HSIL组中PYCR2及CA125的阳性检出率均高于正常子宫颈组(χ^(2)值分别为34.98、51.03,P<0.05);LSIL组及HSIL组中ATG-12的阳性检出率低于正常子宫组(χ^(2)值为61.83,P<0.05);相关性分析结果显示,PYCR2及CA125的阳性检出率与SIL病理分级(LSIL与HSIL)呈显著正相关关系(r=0.321,P<0.05;r=0.387,P<0.05),ATG-12阳性率与SIL病理分级呈显著负相关关系(r=-0.429,P<0.05)。PYCR2、CA125、ATG-12三者联合诊断SIL的AUC为0.996。结论SIL中PYCR2、CA125以及ATG-12的表达水平发生改变,三种指标联合预测模型效果良好,可能作为SIL诊断评估的标志物。Objective To investigate the value of combined detection of pyrroline-5-carbohydrate antigen 2(PYCR2),carbohydrate antigen 125(CA125),and autophagy-related protein-12(ATG-12)in the diagnosis of squamous intraepithelial lesion(SIL)of the uterine cervix.Methods A total of 336 cervical biopsy paraffin-embedded specimens were collected at Nantong Tumor Hospital from January 2020 to December 2021,including 131 cases of normal cervix,91 cases of low grade squamous intraepithelial lesions(LSIL),and 114 cases of high grade squamous intraepithelial lesions(HSIL).The expression levels of PYCR2,CA125,and ATG-12 in each group were detected,and the correlation of PYCR2,CA125,and ATG-12 with SIL was analyzed.The diagnostic value of the combined detection of these three markers in SIL was also evaluated.Results The positive detection rates of PYCR2 and CA125 in the LSIL and HSIL groups were significantly higher than those in the normal cervical group(χ^(2)=34.98 and 51.03,respectively,P<0.05).The positive detection rate of ATG-12 in the LSIL and HSIL groups was significantly lower than that in the normal cervical group(χ^(2)=61.83,P<0.05).Correlation analysis showed that the positive detection rates of PYCR2 and CA125 were significantly positively correlated with SIL pathological grading(LSIL and HSIL)(r=0.321 and 0.387,respectively,P<0.05),while the positive rate of ATG-12 was significantly negatively correlated with SIL pathological grading(r=-0.429,P<0.05).The AUC of the combined diagnosis of SIL using PYCR2,CA125,and ATG-12 was 0.996.Conclusion The expression levels of PYCR2,CA125,and ATG-12 are altered in SIL,and the combined diagnostic model of these three markers shows good performance.They may serve as potential biomarkers for the diagnosis and evaluation of SIL.

关 键 词：子宫颈肿瘤 鳞状上皮内病变 诊断效能 

分 类 号：R179[医药卫生—妇幼卫生保健]