Regulation of Yield and Eating and Cooking Quality in Rice Through Seed Albumin OsRAL5  

作　　者：ZHAO Chunfang HE Lei GUAN Ju LUO Lei ZHAO Ling ZHOU Lihui YAO Shu CHEN Tao ZHU Zhen ZHAO Qingyong WANG Cailin ZHANG Yadong 

机构地区：[1]Key Laboratory of Jiangsu Province for Agrobiology/East China Branch of National Center of Technology Innovation for Saline-Alkali Tolerant Rice/Institute of Food Crops,Jiangsu Academy of Agricultural Science,Nanjing 210014,China

出　　处：《Rice science》2025年第1期6-10,I0010-I0016,共12页水稻科学(英文版)

基　　金：supported by the Natural Science Foundation of Jiangsu Province,China(Grant No.BK20211137);China Agriculture Research System(Grant No.CARS-01)。

摘　　要：Seed storage proteins and their abundance are closely related to the formation of rice quality and grain size.A better understanding of the molecular basis of seed storage proteins will provide important information for developing new rice breeding strategies.In this study,we report that a seed storage protein albumin,named OsRAL5,positively regulates rice quality and grain size.OsRAL5 is specifically expressed during the grain-filling stage,suggesting its key role in regulating seed development.Gene editing of OsRAL5 using the CRISPR/Cas9 system diminished grain size and weight,resulting in the development of white-core endosperm and a reduction in eating and cooking quality(ECQ).Conversely,the endosperm appearance became transparent,and both grain weight and ECQ improved in the OsRAL5 over-expressed lines.The major seed storage components differed significantly between the OsRAL5-edited(dOsRAL5-TS)and OsRAL5-overexpressed(OsRAL5-OE)lines compared with the wild type.The protein and total lipid contents both decreased in the dOsRAL5-TS lines and increased in the OsRAL5-OE lines.Collectively,the over-expression of OsRAL5 significantly increased nutritional content and simultaneously improved yield and ECQ.These results imply that OsRAL5 might be a promising candidate gene for breeding super rice varieties with increased yield potential and superior quality.

关 键 词：CRISPR/Cas9 BREEDING ALBUMIN 

分 类 号：S511[农业科学—作物学] TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]