Disruption of Energy Metabolism and Reactive Oxygen Species Homeostasis in Honglian Type-Cytoplasmic Male Sterility(HL-CMS)Rice Pollen  

作　　者：WANG Mingyue ZHAO Weibo FENG Xiaoya CHEN Yi LI Junhao FU Jinmei YAN Yingchun CHU Zhaohui HUANG Wenchao 

机构地区：[1]State Key Laboratory of Hybrid Rice,Key Laboratory for Research and Utilization of Heterosis in Indica Rice,the Ministry of Agriculture and Rural Affairs/Department of Genetics,College of Life Sciences,Wuhan University,Wuhan 430072,China

出　　处：《Rice science》2025年第1期81-93,I0056-I0058,共16页水稻科学(英文版)

基　　金：supported by the National Natural Science Foundation of China(Grant No.32472185);the Key Research and Development Program of Hubei Province,China(Grant No.2022BFE003);the Hubei Agriculture Science and Technology Innovation Center program,and the National Rice Industry Technology System,China(Grant No.CARS-01-07).

摘　　要：Honglian type-cytoplasmic male sterility(HL-CMS)is caused by the inter-communication between the nucleus and mitochondria.However,the mechanisms by which sterility genes regulate metabolic alterations and changes in mitochondrial morphology in the pollen of HL-CMS remain unclear.In this study,we compared the morphological differences between the pollen of the male sterile line YA and the near-isogenic line NIL-Rf6 using hematoxylin-eosin staining and 4ʹ,6-diamidino-2-phenylindole(DAPI)staining.HL-CMS is characterized by gametophytic sterility,where the aborted pollen grains are empty,and the tapetal layer remains intact.Transmission electron microscopy was employed to observe mitochondrial morphological changes at the microspore stage,revealing significant mitochondrial alterations,characterized by the formation of'large spherical mitochondria',occurred at the binucleate stage in the YA line.Additionally,metabolomics analysis revealed decreased levels of metabolites associated with the carbohydrate and flavonoid pathways.Notably,the decrease in flavonoids was found to contribute to an elevation in reactive oxygen species(ROS)levels.Therefore,we propose a model in which rice fertility is modulated by the levels of pollen carbohydrates and flavonoid metabolites,with impaired mitochondrial energy production and reduced flavonoid biosynthesis as the main causes of ROS accumulation and pollen abortion in rice.

关 键 词：cytoplasmic male sterility POLLEN reactive oxygen species RICE mitochondrial metabolite 

分 类 号：Q94[生物学—植物学]