Mixture of Bacillus Amyloliquefaciens and Bacillus Pumilus Modulates Community Structures of Rice Rhizosphere Soil to Suppress Rice Seedling Blight  

作　　者：JIANG Nan QIU Jiehua TIAN Dagang SHI Huanbin LIU Zhiquan WEN Hui XIE Shuwei CHEN Huizhe WU Meng KOU Yanjun 

机构地区：[1]State Key Laboratory of Rice Biology and Breeding,China National Rice Research Institute,Hangzhou 311400,China [2]Biotechnology Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China [3]State Key Laboratory of Soil and Sustainable Agriculture,the Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China

出　　处：《Rice science》2025年第1期118-130,I0067-I0070,共17页水稻科学(英文版)

基　　金：supported by the Zhejiang Provincial Natural Science Foundation,China(Grant No.LQ24C010007);Zhejiang Science and Technology Major Program on Rice New Variety Breeding,China(Grant No.2021C02063);the Agricultural Sciences and Technologies Innovation Program,China(Grant No.CAAS-CSCB-202301);the Key Projects of Zhejiang Provincial Natural Science Foundation,China(Grant No.LZ23C130002);the Youth Innovation Program of Chinese Academy of Agricultural Sciences(Grant No.Y2023QC22);the Joint Open Competitive Project of the Yazhou Bay Seed Laboratory and China National Seed Company Limited(Grant Nos.B23YQ1514 and B23CQ15EP);the External Cooperation Projects of Biotechnology Research Institute,Fujian Academy of Agricultural Sciences,China(Grant No.DWHZ2024-07).

摘　　要：Rice seedling blight,caused by various fungi,including Fusarium oxysporum,poses a severe threat to rice production.As awareness grows regarding the environmental and safety hazards associated with the application of fungicides for managing rice seedling blight,there has been a shift in focus towards biological control agents.In this study,we isolated biocontrol bacteria from paddy fields that significantly inhibited the growth of F.oxysporum in vitro and identified the strains as Bacillus amyloliquefaciens T40 and Bacillus pumilus T208.Additionally,our findings indicated that the combined application of these Bacillus strains in soil was more effective in reducing the incidence of rice seedling blight than their individual use.Analysis of 16S and internal transcribed spacer rRNA gene sequencing data revealed that the mixture of the T40 and T208 strains exhibited the lowest average clustering coefficients,which were negatively correlated with the biomass of F.oxysporum-inoculated rice seedlings.Furthermore,this mixture led to higher stochastic assembly(average|βNTI|<2)and reduced selection pressures on rice rhizosphere bacteria compared with individual strain applications.The mixture of the T40 and T208 strains also significantly increased the expression of defense-related genes.In conclusion,the mixture of the T40 and T208 strains effectively modulates microbial community structures,enhances microbial network stability,and boosts the resistance against rice seedling blight.Our study supports the development and utilization of biological resources for crop protection.

关 键 词：application strategy disease control disease resistance microbial community structure microbial community assembly process Oryza sativa 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] S154.3[农业科学—植物保护]