不同产羔数藏羊卵巢组织中GDF9基因mRNA表达分析  

Analysis of GDF9 Gene mRNA Expression in Ovarian Tissues of Tibetan Sheep with Different Lambing Capacities

作　　者：金雲鑫 孙武 马世科 金夏阳 马玉红 周玉青 王维英 Jin Yunxin;Sun Wu;Ma Shike;Jin Xiayang;Ma Yuhong;Zhou Yuqing;Wang Weiying(Qinghai Academy of Animal Husbandry and Veterinary Sciences,Xining,810016,Qinghai;Haibei Tibetan Autonomous Prefecture Plateau Ecological Animal Husbandry Science and Technology Demonstration Park Management Committee,Haibei,812200,Qinghai)

机构地区：[1]青海大学畜牧兽医科学院,青海西宁810016 [2]海北藏族自治州高原生态畜牧业科技示范园管委会,青海海北812200

出　　处：《中国畜禽种业》2025年第1期32-39,共8页The Chinese Livestock and Poultry Breeding

基　　金：国家现代农业产业技术体系资助(CARS-39-35);省部共建三江源生态和高原农牧业国家重点实验室自主研究项目(2023-ZZ-11);青海省科技成果转化专项(2023-NK-132);青海省中青年科技人才托举工程(2023QHSKXRCTJ17)。

摘　　要：该研究旨在通过比较高产羔与低产羔藏羊卵巢组织中GDF9 mRNA表达水平,揭示GDF9基因在羊繁殖能力中的潜在作用,从而为提高藏羊的繁殖效率提供基因层面的理论依据。研究选取单胎和多胎藏羊各10只,利用定量PCR技术进行GDF9 mRNA表达水平的检测。结果显示,产双羔组藏羊卵巢组织中的GDF9基因表达量显著高于产单羔组羊卵巢组织(P<0.05)。可见,GDF9基因在藏羊的繁殖过程中可能发挥重要作用,与其产羔能力密切相关。This study aims to reveal the potential role of the GDF9 gene in the reproductive capacity of sheep by comparing the mRNAexpression levels of GDF9 in the ovarian tissues of high-fecundity and low-fecundity Tibetan sheep.The findings provide a genetic basis for improving the reproductive efficiency of Tibetan sheep.In this study,10 single-and multiple-born Tibetan sheep each were selected for the detection of GDF9 mRNA expression levels using quantitative PCR.The results show that the expression level of the GDF9 gene in the ovarian tissues of the high-fecundity group is significantly higher than that in the low-fecundity group(P<0.05).The conclusion suggests that the GDF9 gene may play an important role in the reproductive process of Tibetan ewes and is closely related to their fecundity.

关 键 词：藏羊 GDF9基因 卵巢组织 定量实时PCR 

分 类 号：S831[农业科学—畜牧学]