Transportation engineering for enhanced production of plant natural products in microbial cell factories  

作　　者：Yimeng Zuo Minghui Zhao Yuanwei Gou Lei Huang Zhinan Xu Jiazhang Lian 

机构地区：[1]Key Laboratory of Biomass Chemical Engineering of Ministry of Education&National Key Laboratory of Biobased Transportation Fuel Technology,College of Chemical and Biological Engineering,Zhejiang University,Hangzhou,310027,China [2]ZJU-Hangzhou Global Scientific and Technological Innovation Center,Zhejiang University,Hangzhou,310000,China

出　　处：《Synthetic and Systems Biotechnology》2024年第4期742-751,共10页合成和系统生物技术(英文)

基　　金：supported by Fundamental Research Funds for the Zhejiang Provincial Universities(226-2023-00015);National Natural Science Foundation of China(22278361,32200052,and 32300053);China Postdoctoral Science Foundation(2023M733096);National Key Research and Development Program of China(2018YFA0901800 and 2021YFC2103200).

摘　　要：Plant natural products(PNPs)exhibit a wide range of biological activities and have essential applications in various fields such as medicine,agriculture,and flavors.Given their natural limitations,the production of high-value PNPs using microbial cell factories has become an effective alternative in recent years.However,host metabolic burden caused by its massive accumulation has become one of the main challenges for efficient PNP production.Therefore,it is necessary to strengthen the transmembrane transport process of PNPs.This review introduces the discovery and mining of PNP transporters to directly mediate PNP transmembrane transportation both intracellularly and extracellularly.In addition to transporter engineering,this review also summarizes several auxiliary strategies(such as small molecules,environmental changes,and vesicles assisted transport)for strengthening PNP transportation.Finally,this review is concluded with the applications and future perspectives of transportation engineering in the construction and optimization of PNP microbial cell factories.

关 键 词：Plant natural products Transportation engineering Transporters Microbial cell factories 

分 类 号：Q93[生物学—微生物学]