Heterologous activation and metabolites identification of the pks7 gene cluster from Saccharopolyspora erythraea  

作　　者：Hao Tang Xingchi Yang Wenzong Wang Xingjun Cui Wenping Wei Jing Wu Peng Sun Bang-Ce Ye 

机构地区：[1]Institute of Engineering Biology and Health,Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals,College of Pharmaceutical Sciences,Zhejiang University of Technology,Hangzhou,310014,China [2]School of Pharmacy,Naval Medical University,Shanghai,200433,China [3]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,China

出　　处：《Synthetic and Systems Biotechnology》2024年第4期828-833,共6页合成和系统生物技术(英文)

基　　金：support from the National Key Research and Development Program of China(Nos.2018YFA0900404 and 2022YFC2804105);the National Natural Science Foundation of China(No.82273849).

摘　　要：The microbial genome remains a huge treasure trove for the discovery of diverse natural products.Saccharopolyspora erythraea NRRL23338,the industry producer of erythromycin,has a dozen of biosynthetic gene clusters whose encoding products are unidentified.Heterologous expression of one of the polyketide clusters pks7 in Streptomyces albus B4 chassis resulted in the characterization of its function responsible for synthesizing both 6-methylsalicyclic acid and 6-ethylsalicyclic acid.Meanwhile,two new 6-ethylsalicyclic acid ester derivatives were isolated as shunt metabolites.Their structures were identified by comprehensive analysis of MS and NMR experiments.Putative functions of genes within the pks7 BGC were also discussed.

关 键 词：Heterologous expression Saccharopolyspora erythraea 6-Methylsalicyclic acid 6-Ethylsalicyclic acid 6-Ethylsalicyclic acid ester derivatives Flavonoid 

分 类 号：O62[理学—有机化学]